製品の概要

  • 製品名Anti-TNXB antibody
    TNXB 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to TNXB
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Fragment of full length protein (Human) amino acids 1-673.

  • ポジティブ・コントロール
    • TNXB transfected 293T cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab67586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight of full length protein: 464 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Appears to mediate interactions between cells and the extracellular matrix. Substrate-adhesion molecule that appears to inhibit cell migration. Accelerates collagen fibril formation. May play a role in supporting the growth of epithelial tumors.
    • 組織特異性Highly expressed in fetal adrenal, in fetal testis, fetal smooth, striated and cardiac muscle. Isoform XB-short is only expressed in the adrenal gland.
    • 関連疾患Tenascin-X deficiency (TNXD) [MIM:606408]: TNXD leads to an Ehlers-Danlos-like syndrome characterized by hyperextensible skin, hypermobile joints, and tissue fragility. Tenascin-X-deficient patients, however, lack atrophic scars, a major diagnostic criteria for classic Ehlers-Danlos. Delayed wound healing, which is also common in classic EDS, is only present in a subset of patients. Note=The disease is caused by mutations affecting the gene represented in this entry.
    • 配列類似性Belongs to the tenascin family.
      Contains 19 EGF-like domains.
      Contains 1 fibrinogen C-terminal domain.
      Contains 32 fibronectin type-III domains.
    • 発生段階Expression levels are lower in adults than in children.
    • 細胞内局在Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EDS3 antibody
      • Ehlers Danlos like syndrome antibody
      • Growth inhibiting protein 45 antibody
      • Hexabrachion like protein antibody
      • Hexabrachion-like protein antibody
      • HXBL antibody
      • NXB2 antibody
      • Tenascin X precursor antibody
      • Tenascin XB antibody
      • Tenascin XB1 antibody
      • Tenascin XB2 antibody
      • Tenascin-X antibody
      • TENX antibody
      • TENX_HUMAN antibody
      • TN X antibody
      • TN-X antibody
      • TNX antibody
      • TNXB antibody
      • TNXB1 antibody
      • TNXB2 antibody
      • TNXBS antibody
      • VUR8 antibody
      • XB antibody
      • XBS antibody
      see all

    Anti-TNXB antibody 画像

    • All lanes : Anti-TNXB antibody (ab67586) at 1/500 dilution

      Lane 1 : TNXB transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 464 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    Anti-TNXB antibody (ab67586) 使用論文

    ab67586 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (tendon)
    Antigen retrieval step None
    Permeabilization No
    Specification tendon
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative zinc buffered formalin
    Username

    Abcam user community

    Verified customer

    投稿 Nov 03 2015

    Thank you for your reply. I have issued your free of charge replacement with ab24718 on order ***.

    DISCOUNT CODE: ***
    Expiration date: December 6th, 2012

    I am very pleased to hear you would like to accept our offer and test ab8...

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    Thank you for your reply.

    In order to qualify for the testing discount program, the antibody would need to be purchased for full price. I would be happy to send you this antibody as a free of charge replacement. Alternatively, If you would li...

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    Thank you for your reply. I am sorry that this antibody is still not working in WB. Yes, we are happy to send you a free of charge replacement antibody. If you have an antibody to TNXB that is working for you, I can send you a replacement with an antib...

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    Thank you for your reply. I hope that the 4-12% gel will help to visualize the larger molecular weight bands. If not, please let me know and I will be happy to offer you a free of charge replacement with an alternative antibody, or a credit or refund.

    Thank you for your reply. This antibody has not been validated for use on mouse samples. It is possible that it may cross-react, but we do not have any information about how it performs on mouse cells.

    In human samples, we observe an ˜80 kDa ...

    Read More

    Thank you for contacting us. I am sorry that this antibody is not giving any signal in your experiment. I would be happy to help you further if you could please provide some additional information?

    1) What application were you working in (WB,...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"