製品の概要

  • 製品名
    Anti-TNRC6B antibody
  • 製品の詳細
    Rabbit polyclonal to TNRC6B
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 180-229 (LQSESGTAPV WSKSTPPAPD NGTSAWGEPN ESSPGWGEMD DTGASTTGWG) of human TNRC6B (NP_001020014).

  • ポジティブ・コントロール
    • Human fetal muscle lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified by peptide affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 109 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    TNRC6B belongs to the GW182 family. It contains one RRM (RNA recognition motif) domain. TNRC6B plays a role in RNA mediated gene silencing by both micro RNAs (miRNAs) and short interfering RNAs (siRNAs). It is required for miRNA dependent translational repression and siRNA dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. There are three named isoforms.
  • 細胞内局在
    Cytoplasm, P-body. Mammalian P-bodies are also known as GW bodies, GWBs.
  • 参照データベース
  • 別名
    • KIAA1093 antibody
    • TNRC 6B antibody
    • Trinucleotide repeat containing 6B antibody
    • Trinucleotide repeat containing gene 6B protein antibody

画像

  • Anti-TNRC6B antibody (ab84308) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 109 kDa
    Observed band size : 109 kDa

プロトコール

参考文献

ab84308 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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