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RabMAb

Anti-TNPO3 抗体 [EPR5264] (ab109386)

製品の概要

  • 製品名
    Anti-TNPO3 antibody [EPR5264]
    TNPO3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5264] to TNPO3
  • アプリケーション
    適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C terminal of Human TNPO3.

  • ポジティブ・コントロール
    • HeLa, Jurkat, Ramos and HepG2 cell lysates; Human kidney tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 104 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    画像

    • All lanes : Anti-TNPO3 antibody [EPR5264] (ab109386) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 104 kDa
    • ab109386, at 1/100 dilution, staining TNPO3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    参考文献

    This product has been referenced in:
    • Meehan AM  et al. A cyclophilin homology domain-independent role for Nup358 in HIV-1 infection. PLoS Pathog 10:e1003969 (2014). WB . Read more (PubMed: 24586169) »
    • Torella A  et al. Next-generation sequencing identifies transportin 3 as the causative gene for LGMD1F. PLoS One 8:e63536 (2013). WB ; Human . Read more (PubMed: 23667635) »

    See all 2 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (macrophage)
    Specification
    macrophage
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Dec 12 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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