製品の概要

  • 製品名Anti-TNPO3 antibody
    TNPO3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TNPO3
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human, Bird
    交差が予測される動物種: Cow, Xenopus laevis, Non Human Primates
  • 免疫原

    Synthetic peptide derived from within residues 100 - 200 of Human TNPO3.

    (Peptide available as ab71387.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat (Staurosporine Treated for 24hr, 500nM), Y79, Ramos, MEF1

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 104 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

Anti-TNPO3 antibody 画像

  • All lanes : Anti-TNPO3 antibody (ab71388) at 1 µg/ml

    Lane 1 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 104 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab71388 stained MCF7 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71388, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% paraformaldehyde fixed (10 min) HeLa, Hek293 and HepG2 cells at 5µg/ml.

Anti-TNPO3 antibody (ab71388) 使用論文

This product has been referenced in:
  • De Iaco A & Luban J Inhibition of HIV-1 infection by TNPO3 depletion is determined by capsid and detectable after viral cDNA enters the nucleus. Retrovirology 8:98 (2011). WB . Read more (PubMed: 22145813) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Quail Cell lysate - whole cell (fibroblast)
Loading amount 10 µg
Specification fibroblast
Gel Running Conditions Reduced Denaturing (10% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 16 2012

DISCOUNT CODE: ***
Expiration date: March 8th, 2012
Value: ***

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"