製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TNNI3K
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 756-805 (GRSHVAALRS RFELEYALNA RSYAALSQSA GQYSSQGLSL EEMKRSLQYT) of Human TNNI3K (NP_057062).

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    May play a role in cardiac physiology.
  • 組織特異性
    Highly expressed in both adult and fetal heart.
  • 配列類似性
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 10 ANK repeats.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Autophosphorylated.
  • 細胞内局在
    Nucleus. Cytoplasm. Expressed at lower levels in the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cardiac ankyrin repeat kinase antibody
    • cardiac muscle antibody
    • Cardiac troponin I-interacting kinase antibody
    • CARK antibody
    • CMD1FF antibody
    • CMD2A antibody
    • CMH7 antibody
    • cTnl antibody
    • MGC142099 antibody
    • MGC33828 antibody
    • RCM1 antibody
    • Serine/threonine protein kinase TNN13K antibody
    • Serine/threonine-protein kinase TNNI3K antibody
    • TNI3K_HUMAN antibody
    • TNNC1 antibody
    • TNNI3 interacting kinase antibody
    • TNNI3-interacting kinase antibody
    • Tnni3k antibody
    • Troponin 1 antibody
    see all

画像

  • Anti-TNNI3K antibody (ab86564) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa
    Observed band size : 93 kDa

プロトコール

参考文献

This product has been referenced in:
  • Theis JL  et al. TNNI3K mutation in familial syndrome of conduction system disease, atrial tachyarrhythmia and dilated cardiomyopathy. Hum Mol Genet N/A:N/A (2014). WB ; Mouse . Read more (PubMed: 24925317) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Dog Tissue lysate - whole (heart)
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Glen Pyle

Verified customer

投稿 Sep 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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