製品の概要

  • 製品名Anti-TNNI1 antibody [12F10]
    TNNI1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [12F10] to TNNI1
  • 特異性Detects only slow Troponin I from human skeletal muscle. Cross-reaction of this antibody with cardiac troponin I is less then 0.05%.
  • アプリケーション適用あり: Sandwich ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified) (Human).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名12F10
  • ミエローマSp2/0
  • アイソタイプIgG2b
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [7G2] to Troponin I fast + slow skeletal muscle (HRP) (ab24464). Use as Capture Antibody.
AP Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

ターゲット情報

  • 機能Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 組織特異性Highest levels observed in human skeletal muscle (e.g. gastrocnemious muscle), differentiated cultures of primary human muscle cells and rhabdomyosarcoma cells cultured in low serum medium. Expressed in C2 muscle cell myoblasts and myotubes.
  • 配列類似性Belongs to the troponin I family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2700018B22Rik antibody
    • AI747285 antibody
    • DKFZp451O223 antibody
    • si:dz182n13.3 antibody
    • slow skeletal muscle antibody
    • slow-twitch isoform antibody
    • SSTNI antibody
    • TNN1 antibody
    • Tnni1 antibody
    • TNNI1_HUMAN antibody
    • Troponin I antibody
    • Troponin I slow skeletal muscle antibody
    • Troponin I slow twitch isoform antibody
    • troponin I type 1 (skeletal, slow) antibody
    • Troponin I, slow isoform antibody
    see all

Anti-TNNI1 antibody [12F10] 画像

  • All lanes : Anti-TNNI1 antibody [12F10] (ab8293) at 1 µg/ml

    Lane 1 : 40ug human muscle lysate (whole tissue)
    Lane 2 : 40ug human muscle lysate (whole tissue)
    Lane 3 : 40ug human muscle lysate (whole tissue)
    Lane 4 : 40ug human muscle lysate (whole tissue)
    Lane 5 : 40ug human muscle lysate (whole tissue)
    Lane 6 : 40ug human muscle lysate (whole tissue)
    Lane 7 : 40ug rat adipose lysate (whole tissue)

    Secondary
    HRP donkey anti-mouse HRP
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Observed band size : 22 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-TNNI1 antibody [12F10] (ab8293) 使用論文

This product has been referenced in:
  • Zhou Y  et al. Pitx2 regulates myosin heavy chain isoform expression and multi-innervation in extraocular muscle. J Physiol 589:4601-14 (2011). Read more (PubMed: 21727215) »
  • Groshong JS  et al. Calpain activation impairs neuromuscular transmission in a mouse model of the slow-channel myasthenic syndrome. J Clin Invest 117:2903-12 (2007). WB ; Human . Read more (PubMed: 17853947) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (skeletal muscle)
Loading amount 30 µg
Specification skeletal muscle
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Aug 19 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Muscle biopsy material)
Loading amount 40 µg
Specification Muscle biopsy material
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5%
Username

Abcam user community

Verified customer

投稿 Oct 26 2006

Thank you for your enquiry. Unfortunately we have not determined the epitopes. However, the antibodies are forming a pair in ELISA. Recommended pair is 12F10 - 7G2 (capture - conjugate).

Unfortunately, we do not sell ab8294 conjugated to HRP. You will need to conjugate this antibody to HRP yourself in order to use it in a sandwich ELISA. ab9920 (human skeletal muscle Troponin I) should be suitable for use as a positive control (we can...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"