製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to TNIP1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    TNIP1 full-length human protein (NP_006049)

  • ポジティブ・コントロール
    • TNIP1 transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 72 kDa).

ターゲット情報

  • 機能
    Interacts with zinc finger protein A20/TNFAIP3 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal (By similarity). Increases cell surface CD4(T4) antigen expression. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production.
  • 組織特異性
    Ubiquitous. Strongly expressed in peripheral blood lymphocytes, spleen and skeletal muscle, and is weakly expressed in the brain.
  • 細胞内局在
    Cytoplasm. Nucleus. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ABIN 1 antibody
    • HIV-1 Nef-interacting protein antibody
    • hVAN antibody
    • KIAA0113 antibody
    • Naf1 antibody
    • Nef-associated factor 1 antibody
    • Nip40-1 antibody
    • TNFAIP3 interacting protein 1 antibody
    • TNFAIP3-interacting protein 1 antibody
    • TNIP-1 antibody
    • Tnip1 antibody
    • TNIP1_HUMAN antibody
    • VAN antibody
    • Virion-associated nuclear shuttling protein antibody
    see all

画像

  • All lanes : Anti-TNIP1 antibody (ab70152) at 1/500 dilution

    Lane 1 : TNIP1 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 72 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab70152 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Dec 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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