The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
1/500 - 1/1000.
Acts as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation.
Expressed at high levels in the spleen, lymph node, thymus, thyroid, bone marrow and placenta. Expressed at high levels both in various tumor tissues, unstimulated and cytokine-activated cultured cells. Expressed at low levels in the spinal cord, ovary, lung, adrenal glands, heart, brain, testis and skeletal muscle.
Belongs to the TNFAIP8 family.
Expressed at high levels in the fetal liver, lung and kidney.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using TNFAIP8 antibody (ab64988) at 1/50 dilution, in the presence (right panel) and absence (left panel) of immunising peptide. Antigen retrieval was performed by boiling in a pressure cooker for 2-3 min. A Polymer system was used for signal enhancing. Stained using DAB.
Chen L et al. Association between the expression levels of tumor necrosis factor-a-induced protein 8 and the prognosis of patients with gastric adenocarcinoma. Exp Ther Med12:238-244 (2016).
Read more (PubMed: 27347043) »
Xing B & Ren C Tumor-suppressive miR-99a inhibits cell proliferation via targeting of TNFAIP8 in osteosarcoma cells. Am J Transl Res8:1082-90 (2016).
Read more (PubMed: 27158394) »