The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5 µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 90 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
Belongs to the peptidase C64 family. Contains 7 A20-type zinc fingers. Contains 1 OTU domain.
The A20-type zinc fingers mediate the ubiquitin ligase activity. The OTU domain mediates the deubiquitinase activity.
ab93868, at 1 µg/ml, detecting TNFAIP3 in HeLa whole cell lysate by WB of IP.
Lane 1: IP with ab93868 at 3 µg/mg of lysate.
Lane 2: Control IgG.
1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence an with exposure time of 30 seconds.