製品の概要

  • 製品名Anti-TNFAIP3 antibody [59A426]
    TNFAIP3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [59A426] to TNFAIP3
  • アプリケーション適用あり: IHC-P, Flow Cyt, WB, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fusion protein of full length TNFAIP3 (Human).

  • エピトープThe epitope has been mapped to the C-terminal portion of A20, amino acids 440-790.
  • ポジティブ・コントロール
    • Whole cell lysate from Daudi cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 19637364
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 70 kDa.
ICC/IF Use at an assay dependent concentration.
IP Use a concentration of 1 - 2 µg/ml.

ターゲット情報

  • 機能Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
  • 配列類似性Belongs to the peptidase C64 family.
    Contains 7 A20-type zinc fingers.
    Contains 1 OTU domain.
  • ドメインThe A20-type zinc fingers mediate the ubiquitin ligase activity.
    The OTU domain mediates the deubiquitinase activity.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A20 antibody
    • MGC104522 antibody
    • MGC138687 antibody
    • MGC138688 antibody
    • OTU domain containing protein 7C antibody
    • OTU domain-containing protein 7C antibody
    • OTUD7C antibody
    • Putative DNA binding protein A20 antibody
    • Putative DNA-binding protein A20 antibody
    • TNAP3_HUMAN antibody
    • TNF alpha-induced protein 3 antibody
    • TNFA1P2 antibody
    • TNFAIP 3 antibody
    • TNFAIP3 (A20) antibody
    • TNFAIP3 antibody
    • Tumor necrosis factor alpha induced protein 3 antibody
    • Tumor necrosis factor alpha-induced protein 3 antibody
    • Tumor necrosis factor induced protein 3 antibody
    • Tumor necrosis factor inducible protein A20 antibody
    • tumor necrosis factor, alpha-induced protein 3 antibody
    • Zinc finger protein A20 antibody
    see all

Anti-TNFAIP3 antibody [59A426] 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue labelling TNFAIP3 with ab13597 at 5µg/ml. Staining was enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

  • Anti-TNFAIP3 antibody [59A426] (ab13597) at 4 µg/ml + Jurkat cell lysate
  • All lanes : Anti-TNFAIP3 antibody [59A426] (ab13597) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa,34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Overlay histogram showing HepG2 cells stained with ab13597 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13597, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HepG2 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-TNFAIP3 antibody [59A426] (ab13597) 使用論文

This product has been referenced in:
  • Altonsy MO  et al. Context-dependent cooperation between nuclear factor ?B (NF-?B) and the glucocorticoid receptor at a TNFAIP3 intronic enhancer: a mechanism to maintain negative feedback control of inflammation. J Biol Chem 289:8231-9 (2014). WB ; Human . Read more (PubMed: 24500711) »
  • Haemmig S  et al. miR-125b controls apoptosis and temozolomide resistance by targeting TNFAIP3 and NKIRAS2 in glioblastomas. Cell Death Dis 5:e1279 (2014). WB . Read more (PubMed: 24901050) »

See all 11 Publications for this product

Product Wall

There is data to suggest that there is a 58kD band present in many of the samples tested with this antibody. This was tested externally so I am sorry I am not able to provide the data itself. Analysis of A20 using this antibody in WB showed as a stack ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116, HT29 and Caco2 colon cancer cell lines)
Loading amount 30 µg
Specification HCT116, HT29 and Caco2 colon cancer cell lines
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in TBST
Username

Abcam user community

Verified customer

投稿 Nov 07 2006

Thank you for your enquiry. Our two A20 antibodies raised in mice have been tested for Western blotting on human samples. Human A20 is 89% homologous with the sequence for mouse A20. So there is a good chance these antibodies will recognize mouse A2...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"