製品の概要

  • 製品名Anti-TNFAIP1 antibody
    TNFAIP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TNFAIP1
  • 特異性This antibody detects endogenous levels of total TNFAIP1 protein.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthesized peptide derived from an internal region of human TNFAIP1.

  • ポジティブ・コントロール
    • Extracts from HUVEC cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Store at -20°C. Stable for 12 months at -20°C
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: 1/5000.
    WB: 1/500 - 1/1000. Detects bands of approximately 34, 60 and 68 kDa (predicted molecular weight: 36 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD2) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.
    • パスウェイProtein modification; protein ubiquitination.
    • 配列類似性Belongs to the BACURD family.
      Contains 1 BTB (POZ) domain.
    • 翻訳後修飾Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.
    • 細胞内局在Cytoplasm. Nucleus. Endosome. Colocalizes with RHOB in endosomes.
    • Information by UniProt
    • 参照データベース
    • 別名
      • alpha-induced protein 1 antibody
      • BACD2_HUMAN antibody
      • BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 antibody
      • BTB/POZ domain-containing protein TNFAIP1 antibody
      • endothelial antibody
      • hBACURD2 antibody
      • Protein B12 antibody
      • tnfaip1 antibody
      • Tumor necrosis factor alpha induced protein 1 (endothelial) antibody
      • Tumor necrosis factor alpha induced protein 1 antibody
      • Tumor necrosis factor antibody
      see all

    Anti-TNFAIP1 antibody 画像

    • All lanes : Anti-TNFAIP1 antibody (ab72626) at 1/500 dilution

      Lane 1 : Extracts from HUVEC cells, plus PMA (125ng/ml, 30mins)
      Lane 2 : Extracts from HUVEC cells, plus serum (20%, 30mins)
      Lane 3 : Extracts from HUVEC cells, plus PMA (125ng/ml, 30mins) and serum (20%, 30mins) plus immunizing peptide

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 36 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 and 68 kDa. We are unsure as to the identity of these extra bands.

    Anti-TNFAIP1 antibody (ab72626) 使用論文

    ab72626 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab72626.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"