製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TNFAIP1
  • 特異性
    This antibody detects endogenous levels of total TNFAIP1 protein.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthesized peptide derived from an internal region of human TNFAIP1.

  • ポジティブ・コントロール
    • Extracts from HUVEC cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Store at -20°C. Stable for 12 months at -20°C
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    ELISA: 1/5000.
    WB: 1/500 - 1/1000. Detects bands of approximately 34, 60 and 68 kDa (predicted molecular weight: 36 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD2) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 配列類似性
      Belongs to the BACURD family.
      Contains 1 BTB (POZ) domain.
    • 翻訳後修飾
      Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.
    • 細胞内局在
      Cytoplasm. Nucleus. Endosome. Colocalizes with RHOB in endosomes.
    • Information by UniProt
    • 参照データベース
    • 別名
      • alpha-induced protein 1 antibody
      • BACD2_HUMAN antibody
      • BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 antibody
      • BTB/POZ domain-containing protein TNFAIP1 antibody
      • endothelial antibody
      • hBACURD2 antibody
      • Protein B12 antibody
      • tnfaip1 antibody
      • Tumor necrosis factor alpha induced protein 1 (endothelial) antibody
      • Tumor necrosis factor alpha induced protein 1 antibody
      • Tumor necrosis factor antibody
      see all

    Anti-TNFAIP1 antibody 画像

    • All lanes : Anti-TNFAIP1 antibody (ab72626) at 1/500 dilution

      Lane 1 : Extracts from HUVEC cells, plus PMA (125ng/ml, 30mins)
      Lane 2 : Extracts from HUVEC cells, plus serum (20%, 30mins)
      Lane 3 : Extracts from HUVEC cells, plus PMA (125ng/ml, 30mins) and serum (20%, 30mins) plus immunizing peptide

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 36 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 and 68 kDa. We are unsure as to the identity of these extra bands.

    プロトコール

    Anti-TNFAIP1 antibody (ab72626) 使用論文

    ab72626 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab72626.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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