The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The antibody is an agonistic antibody useful for cell culture experiments and for detection of TNF-R II by FACS analysis.
Furthermore it can be used for immunoprecipitation and histology on frozen sections.
The reactivity of the antibody with cellbound or soluble TNF-Receptor is not inhibited by high concentrations of mouse TNF-?.
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Contains 4 TNFR-Cys repeats.
Phosphorylated; mainly on serine residues and with a very low level on threonine residues. A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.