アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TNF Receptor II (C terminal).
(Peptide available as
For best results in ICC/IF please use 100% Methanol fixation.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Our Abpromise guarantee covers the use of ab109322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 73 kDa (predicted molecular weight: 48 kDa).Can be blocked with TNF Receptor II peptide (ab192471).|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Antigen retrieval is recommended.|
|ICC||1/250 - 1/500.
For best results use 100% Methanol fixation.
|Flow Cyt||1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
ab109322 staining TNF Receptor II in SW480 cells. The cells were fixed with 100% methanol. In our hands PFA fixed cells did not produce good results with this antibody. Nuclear DNA was labelled in blue with DAPI.
Primary: ab109322, 1/1000 dilution
Sample: Jurkat whole cell lysate, 100 ug
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, 1/1000 dilution
Capture antibody: 1:40 dilution (1µg in 1 mg lysate)
Blocking and diluting buffer: 5% NFDM/TBST