The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.
Use a concentration of 2 µg/ml.
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Contains 4 TNFR-Cys repeats.
Phosphorylated; mainly on serine residues and with a very low level on threonine residues. A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
Kim JE et al. Status Epilepticus Induces Vasogenic Edema via Tumor Necrosis Factor-a/ Endothelin-1-Mediated Two Different Pathways. PLoS One8:e74458 (2013).
Read more (PubMed: 24040253) »
Al-Zoubi M et al. Creating a tumor-resistant microenvironment: cell-mediated delivery of TNFa completely prevents breast cancer tumor formation in vivo. Cell Cycle12:480-90 (2013).
Read more (PubMed: 23292149) »