製品の概要

  • 製品名
    Anti-TNF Receptor II antibody
    TNF Receptor II 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TNF Receptor II
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Dog, Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (Mouse) (C terminal).

  • ポジティブ・コントロール
    • Thymus

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.
IP Use a concentration of 2 µg/ml.
IHC-P 1/50.

ターゲット情報

  • 機能
    Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
  • 配列類似性
    Contains 4 TNFR-Cys repeats.
  • 翻訳後修飾
    Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
    A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD120b antibody
    • p75 antibody
    • p75 TNF receptor antibody
    • p75TNFR antibody
    • p80 TNF alpha receptor antibody
    • p80 TNF-alpha receptor antibody
    • Soluble TNFR1B variant 1 antibody
    • TBP-2 antibody
    • TBPII antibody
    • TNF R II antibody
    • TNF R2 antibody
    • TNF R75 antibody
    • TNF-R2 antibody
    • TNF-RII antibody
    • TNFBR antibody
    • TNFR-II antibody
    • TNFR1B antibody
    • TNFR2 antibody
    • TNFR80 antibody
    • TNFRII antibody
    • Tnfrsf1b antibody
    • TNR1B_HUMAN antibody
    • Tumor necrosis factor beta receptor antibody
    • Tumor necrosis factor receptor 2 antibody
    • Tumor necrosis factor receptor superfamily member 1B antibody
    • Tumor necrosis factor receptor type II antibody
    • Tumor necrosis factor-binding protein 2 antibody
    see all

画像

  • ab15563 staining TNF Receptor II at 1/50 dilution in thymus by Immunohistochemistry (FFPE-sections).
  • Anti-TNF Receptor II antibody (ab15563) at 1/1000 dilution + whole cell lysate prepared from human platelets (treated with ADP for 30 minutes) at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 55 kDa


    Exposure time : 2 minutes

    Image courtesy of Dr Mahesh Shivananjappa by Abreview.

    Blocked with 5% milk for 1 hour at 22°C.

    See Abreview

参考文献

This product has been referenced in:
  • Kim JE  et al. Status Epilepticus Induces Vasogenic Edema via Tumor Necrosis Factor-a/ Endothelin-1-Mediated Two Different Pathways. PLoS One 8:e74458 (2013). WB, ICC/IF ; Rat . Read more (PubMed: 24040253) »
  • Al-Zoubi M  et al. Creating a tumor-resistant microenvironment: cell-mediated delivery of TNFa completely prevents breast cancer tumor formation in vivo. Cell Cycle 12:480-90 (2013). Read more (PubMed: 23292149) »

See all 11 Publications for this product

レビューと Q&A

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Filter by Species

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (CD14+)
Loading amount
20 µg
Specification
CD14+
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.6% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Feb 06 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Monocytes from Apheresis)
Specification
Monocytes from Apheresis
Fixation
BD Cytofix/Cytoperm 20 min
Permeabilization
Yes - BD Cytofix/Cytoperm 20 min
Gating Strategy
Monocytes
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Dec 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Platelets)
Loading amount
20 µg
Specification
Platelets
Treatment
ADP for 30 min
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Nov 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (TF-1 cells)
Total protein in input
30 µg
Specification
TF-1 cells
Immuno-precipitation step
Protein A/G
Username

Dr. Alison Taylor

Verified customer

投稿 Nov 08 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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