製品の概要

  • 製品名Anti-TNF Receptor I antibody [H398]
    TNF Receptor I 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [H398] to TNF Receptor I
  • 特異性ab2257 reacts with the extra-cellular part of the TNF-RI. It also reacts with the soluble receptor. Be aware that the antibody competes with TNF-alpha.
  • アプリケーション適用あり: IP, IHC-Fr, Flow Cytmore details
    適用なし: WB
  • 種交差性
    交差種: Human
  • ポジティブ・コントロール
    • Frozen sections: Human lymphnodes. Flow cytometry: HeLa cells. Please note: HeLa cells can differ strongly in TNF-R numbers with some populations having very low numbers.
  • 特記事項


    The reactivity of ab2257 with cellbound TNF-Receptor is minimally inhibited by high concentrations of TNF-alpha.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
IHC-Fr 1/10.
Flow Cyt 1/10.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
    • 関連疾患Familial hibernian fever
      Multiple sclerosis 5
    • 配列類似性Contains 1 death domain.
      Contains 4 TNFR-Cys repeats.
    • ドメインThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
      Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
    • 翻訳後修飾The soluble form is produced from the membrane form by proteolytic processing.
    • 細胞内局在Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD120a antibody
      • FPF antibody
      • MGC19588 antibody
      • p55 antibody
      • p55-R antibody
      • p60 antibody
      • TBP1 antibody
      • TBPI antibody
      • TNF R antibody
      • TNF R55 antibody
      • TNF-R1 antibody
      • TNF-RI antibody
      • TNFAR antibody
      • TNFR-I antibody
      • TNFR1 antibody
      • TNFR55 antibody
      • TNFR60 antibody
      • TNFRI antibody
      • TNFRSF1a antibody
      • TNR1A_HUMAN antibody
      • Tumor necrosis factor receptor 1 antibody
      • Tumor necrosis factor receptor superfamily, member 1A antibody
      • Tumor necrosis factor receptor type 1 antibody
      • Tumor necrosis factor receptor type I antibody
      • Tumor necrosis factor-binding protein 1 antibody
      see all

    Anti-TNF Receptor I antibody [H398] (ab2257) 使用論文

    This product has been referenced in:
    • Mahata B  et al. GBM Derived Gangliosides Induce T Cell Apoptosis through Activation of the Caspase Cascade Involving Both the Extrinsic and the Intrinsic Pathway. PLoS One 10:e0134425 (2015). IP, ICC/IF ; Human . Read more (PubMed: 26226135) »
    • Greco SJ & Rameshwar P Enhancing effect of IL-1alpha on neurogenesis from adult human mesenchymal stem cells: implication for inflammatory mediators in regenerative medicine. J Immunol 179:3342-50 (2007). WB ; Human . Read more (PubMed: 17709551) »

    See all 4 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (brain endothelial cells)
    Loading amount 10 µg
    Specification brain endothelial cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15%
    Username

    Abcam user community

    Verified customer

    投稿 May 30 2007

    Thank you for your enquiry. I am contacting the person who made this data sheet to first confirm that the dilution is 1/10 because that seems rather concentrated to me. I will get back to you as soon as I hear back. Thank you for your patience. I h...

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    Thank you for your question concerning the ab2257. Concerning the source of immunizing protein used to generate this monoclonal we have received the following information: About 220µg affinity-purified receptor material from HL-60 cells containing ...

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    Thank you for your enquiry. Unfortunately this information is not available. If you have any additional questions, please contact us again.

    In principle it could be used for the precipitation of soluble TNF Receptor 1 in serum, however this has not been specifically tested to our knowledge.

    Thanks for your email. Ab2257 is not avaialble conjugated and we are unable to do custom conjugation. If you have any further questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"