製品の概要

  • 製品名
    Anti-TNF Receptor I antibody
    TNF Receptor I 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TNF Receptor I
  • 特異性
    ab58436 detects endogenous levels of total TNF Receptor I protein. This antibody binds to the C-terminal cytoplasmic domain and reacts with three isoforms (1, 2, and 3) of human TNF Receptor I.
  • アプリケーション
    適用あり: ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from an internal sequence of human TNF Receptor I

  • ポジティブ・コントロール
    • IHC-P: Human breast carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
  • 関連疾患
    Familial hibernian fever
    Multiple sclerosis 5
  • 配列類似性
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • ドメイン
    The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
    Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
  • 翻訳後修飾
    The soluble form is produced from the membrane form by proteolytic processing.
  • 細胞内局在
    Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD120a antibody
    • FPF antibody
    • MGC19588 antibody
    • p55 antibody
    • p55-R antibody
    • p60 antibody
    • TBP1 antibody
    • TBPI antibody
    • TNF R antibody
    • TNF R55 antibody
    • TNF-R1 antibody
    • TNF-RI antibody
    • TNFAR antibody
    • TNFR-I antibody
    • TNFR1 antibody
    • TNFR55 antibody
    • TNFR60 antibody
    • TNFRI antibody
    • TNFRSF1a antibody
    • TNR1A_HUMAN antibody
    • Tumor necrosis factor receptor 1 antibody
    • Tumor necrosis factor receptor superfamily, member 1A antibody
    • Tumor necrosis factor receptor type 1 antibody
    • Tumor necrosis factor receptor type I antibody
    • Tumor necrosis factor-binding protein 1 antibody
    see all

画像

  • ab58436, at 1/50 dilution, staining TNF Receptor I in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.

プロトコール

参考文献

This product has been referenced in:
  • Shivakumar P  et al. Preferential TNFa signaling via TNFR2 regulates epithelial injury and duct obstruction in experimental biliary atresia. JCI Insight 2:e88747 (2017). IF ; Mouse . Read more (PubMed: 28289704) »
  • Moutsopoulos NM  et al. Lack of efficacy of etanercept in Sjögren syndrome correlates with failed suppression of tumour necrosis factor alpha and systemic immune activation. Ann Rheum Dis 67:1437-43 (2008). IHC-P ; Human . Read more (PubMed: 18198195) »

See all 2 Publications for this product

レビューと Q&A

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Specification
Spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1 Standard
Permeabilization
No
Blocking step
S Block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 37°C
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投稿 Dec 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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