Anti-TNF Receptor I 抗体 (ab223352)
Key features and details
- Rabbit polyclonal to TNF Receptor I
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TNF Receptor I antibody
TNF Receptor I 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TNF Receptor I -
由来種
Rabbit -
特異性
Detects ~55kDa. Other bands present may be the result of oligomerization, self-aggregation and/or cleavage of the TNF-R1 extracellular domain.
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アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide corresponding to Mouse TNF Receptor I aa 1-100.
Database link: P19438 -
ポジティブ・コントロール
- WB: Mouse liver lysate, Human A549 ICC/IF: HeLa (human epithelial cell line from cervix adenocarcinoma) and HaCaT (human keratinocyte cell line) cells. IHC: Mouse back skin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab223352の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100.
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WB |
1/1000. Predicted molecular weight: 50.5 kDa.
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IHC-P |
1/100.
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特記事項 |
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ICC/IF
1/100. |
WB
1/1000. Predicted molecular weight: 50.5 kDa. |
IHC-P
1/100. |
ターゲット情報
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機能
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. -
関連疾患
Familial hibernian fever
Multiple sclerosis 5 -
配列類似性
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
ドメイン
The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. -
翻訳後修飾
The soluble form is produced from the membrane form by proteolytic processing. -
細胞内局在
Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 7132 Human
- Entrez Gene: 21937 Mouse
- Entrez Gene: 25625 Rat
- Omim: 191190 Human
- SwissProt: P19438 Human
- SwissProt: P25118 Mouse
- SwissProt: P22934 Rat
- Unigene: 279594 Human
see all -
別名
- CD120a antibody
- FPF antibody
- MGC19588 antibody
see all
画像
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Anti-TNF Receptor I antibody (ab223352) at 1/1000 dilution + mouse liver lysate at 15 µg
Secondary
Goat anti-rabbit IgG-HRP at 1/2000 dilution
Predicted band size: 50.5 kDaBlocking: 5% skimmed milk in 1X TBST.
Primary incubation: 2 hours at room temperature
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Anti-TNF Receptor I antibody (ab223352) at 1/1000 dilution + Human A549 at 30 µg
Secondary
Goat Anti-Rabbit IgG HRP at 1/4000 dilution
Predicted band size: 50.5 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking buffer: 5% BSA in TBST.
Other bands ( ~90-100kDa) can be explained by factors such as oligomerization, self-aggregation, cleavage of the TNFR1 extracellular domain, etc (Literature references: doi: 10.3389/fcell.2020.615141, 10.1128/MCB.22.8.2536-2543.2002, 10.1073/pnas.0307981100).
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2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TNF Receptor I (red) using ab223352 at a dilution of 1/100 in ICC/IF (panel B). The secondary antibody is APC Goat Anti-Rabbit used at 1/200 dilution. The nuclear counter stain is DAPI (blue)(panel A). The merged images are shown in panel C.
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2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TNF Receptor I (green) using ab223352 at a dilution of 1/100 in ICC/IF (panel B). The secondary antibody is FITC Goat Anti-Rabbit used at 1/200 diliution. The nuclear counter stain is DAPI (blue)(panel A) used at 1/40,000 dilution. The merged images are shown in panel C.
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Methanol-fixed HaCaT (human keratinocyte cell line) cells stained for TNF Receptor I (green) using ab223352 at a dilution of 1/100 in ICC/IF. The secondary antibody is FITC Goat Anti-Rabbit at a 1/50 dilution.
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Sections of mouse back skin were stained for TNF Receptor I with ab223352 at a dilution of 1/100 in immunohistochemical analysis.
The secondary antibody was FITC Goat Anti-Rabbit (green) at a 1/50 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab223352 は 4 報の論文で使用されています。
- Fischer A et al. Neutrophils direct preexisting matrix to initiate repair in damaged tissues. Nat Immunol 23:518-531 (2022). PubMed: 35354953
- Fan Y et al. Klotho in Osx+-mesenchymal progenitors exerts pro-osteogenic and anti-inflammatory effects during mandibular alveolar bone formation and repair. Signal Transduct Target Ther 7:155 (2022). PubMed: 35538062
- Moujalled D et al. The necroptotic cell death pathway operates in megakaryocytes, but not in platelet synthesis. Cell Death Dis 12:133 (2021). PubMed: 33510145
- Chen X et al. Heparanase induces necroptosis of microvascular endothelial cells to promote the metastasis of hepatocellular carcinoma. Cell Death Discov 7:33 (2021). PubMed: 33597510