製品の概要

  • 製品名
    Anti-TNF Receptor I antibody
    TNF Receptor I 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TNF Receptor I
  • アプリケーション
    適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Monkey
  • 免疫原

    Synthetic peptide corresponding to Mouse TNF Receptor I aa 29-43.

  • ポジティブ・コントロール
    • HeLa heat shocked cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa.
IHC-P 1/1000. Antigen retrieval is not essential but may optimise staining.

Incubate for 1 hour at RT.

Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 17210691

ターゲット情報

  • 機能
    Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
  • 関連疾患
    Familial hibernian fever
    Multiple sclerosis 5
  • 配列類似性
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • ドメイン
    The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
    Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
  • 翻訳後修飾
    The soluble form is produced from the membrane form by proteolytic processing.
  • 細胞内局在
    Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD120a antibody
    • FPF antibody
    • MGC19588 antibody
    • p55 antibody
    • p55-R antibody
    • p60 antibody
    • TBP1 antibody
    • TBPI antibody
    • TNF R antibody
    • TNF R55 antibody
    • TNF-R1 antibody
    • TNF-RI antibody
    • TNFAR antibody
    • TNFR-I antibody
    • TNFR1 antibody
    • TNFR55 antibody
    • TNFR60 antibody
    • TNFRI antibody
    • TNFRSF1a antibody
    • TNR1A_HUMAN antibody
    • Tumor necrosis factor receptor 1 antibody
    • Tumor necrosis factor receptor superfamily, member 1A antibody
    • Tumor necrosis factor receptor type 1 antibody
    • Tumor necrosis factor receptor type I antibody
    • Tumor necrosis factor-binding protein 1 antibody
    see all

Anti-TNF Receptor I antibody 画像

  • Western blot analysis of mouse RAW 264 cell lysate.
  • Ab19139 staining human normal placenta. Staining is localized to cell membrane and secreted.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Anti-TNF Receptor I antibody (ab19139) at 1/1000 dilution + Human 293T whole cell lysate at 30000 cells

    Secondary
    An HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution

    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa (possible non-specific binding),48 kDa (possible non-specific binding).

    This image is courtesy of an anonymous Abreview

    Blocking Step :5% Milk + 1% BSA for 16 hours at 4°C
    Gel Running Conditions : Reduced, Denaturing 12% Bis-Tris

    See Abreview

  • ab19139 staining TNF Receptor I in Human platelet cells by Flow cytometry.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/100 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.

    See Abreview

Anti-TNF Receptor I antibody (ab19139) 使用論文

This product has been referenced in:

See all 54 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Intestinal epithelial cell)
Gel Running Conditions
Reduced Denaturing (6-15% gradient gel)
Loading amount
20 µg
Specification
Intestinal epithelial cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 01 2016

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
100 ng/ml LPS
Specification
mouse hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Nov 16 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Sample
Mouse Cell (hepatocytes)
Specification
hepatocytes
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 19 2014

Our guarantee (www.abcam.com/abpromise) will cover pig reactivity for the TNFR1 antibody ab19139, but we do not have the data that demonstrated cross-reactivity. A customer several years ago used this antibody to stain sections of pig placenta and repo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (RAW264.7)
Specification
RAW264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton + 2% BSA in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Apr 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Specification
Spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1 Standard
Permeabilization
No
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Dec 17 2012

Vielen Dank für Ihre Anfrage.
Die meisten unserer Antikörper wurden nur unter denaturierenden/ reduzierenden WB Bedingungen getestet. Ich möchte daher vorschlagen, die Probe 5 min zu kochen und ein reduzierendes Gel zu verwenden.
Unsere Standa...

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Vielen Dank für Ihren Anruf.

Ich haben den folgenden Artikel gefunden, welcher trotz Trypsinisierung ein gutes Signal bekommt (und das Signal sogar gegen eine nicht-trypsinisierte Probe vergleicht):

http://www.ncbi.nlm.nih.gov/...

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Thank you for contacting us.

We currently only carry this one anti-TNFR FITC conjugated.

OurAnti-TNF Receptor I antibody ab19139 has been tested to react with human tissues in a number of applications. While this product is not ...

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Thanks for your email!

Please let me know if you need anything further.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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