製品の概要

  • 製品名
    Anti-TNF alpha antibody [TN3-19.12]
    TNF alpha 一次抗体 製品一覧
  • 製品の詳細
    Armenian Hamster monoclonal [TN3-19.12] to TNF alpha
  • 特異性
    Cross-reacts with mouse TNF beta.
  • アプリケーション
    適用あり: WB, IP, ELISA, Neutralisingmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Other Immunogen Type corresponding to Mouse TNF alpha. purified recombinant mouse TNF-alpha

  • 特記事項
    For continuous use, store sterile at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 17 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
Neutralising Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 関連疾患
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 配列類似性
    Belongs to the tumor necrosis factor family.
  • 翻訳後修飾
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

Anti-TNF alpha antibody [TN3-19.12] (ab10863) 使用論文

This product has been referenced in:
  • Yang H  et al. TNF Accelerates Death of Mandibular Condyle Chondrocytes in Rats with Biomechanical Stimulation-Induced Temporomandibular Joint Disease. PLoS One 10:e0141774 (2015). Read more (PubMed: 26529096) »
  • Chang JS  et al. Endoplasmic reticulum stress response promotes cytotoxic phenotype of CD8aß+ intraepithelial lymphocytes in a mouse model for Crohn's disease-like ileitis. J Immunol 189:1510-20 (2012). Read more (PubMed: 22753943) »

See all 5 Publications for this product

Product Wall

Thank you for your enquiry. I can confirm that the antibody was raised using hybridoma produced by the fusion of mouse myeloma cells and splenocytes from male Armenian hamster. Therefore, a suitable secondary antibody would be: ab5745 Rabbit po...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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