Anti-TNF alpha 抗体 [T1] (ab8162)


  • 製品名Anti-TNF alpha antibody [T1]
    TNF alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [T1] to TNF alpha
  • 特異性The antibody reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. The antibody inhibits the biological activity of both forms. It does not react with receptor bound TNF-alpha. It can be a useful tool to discriminate between the membrane form of TNF expressed on producer cells and the proteolytically cleaved, soluble TNF-alpha bound to its cognate cell membrane receptors (TNF-RI and TNF-RII).
  • アプリケーション適用あり: Flow Cyt, IP, Inhibition Assay, WBmore details
  • 種交差性
    交差種: Human



Our Abpromise guarantee covers the use of ab8162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Used at a dilution of 1/300 on human brain lysate (see Abreview for further information). 


  • 機能Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 関連疾患Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 配列類似性Belongs to the tumor necrosis factor family.
  • 翻訳後修飾The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

Anti-TNF alpha antibody [T1] (ab8162) 使用論文

ab8162 has not yet been referenced specifically in any publications.

Product Wall

The antibodies are not mapped on the epitopes they would recognize. We do not know whether ab16921 is useful, whereas ab8163 has been characterized more specifically. A combination of our antibodies ab8162 and ab8163 can be used for detection of recept...

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Application Flow Cytometry
Sample Human Cell (HL cell lines and primary human NK cells)
Specification HL cell lines and primary human NK cells
Permeabilization No
Gating Strategy living cell population

Abcam user community

Verified customer

投稿 Sep 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

投稿 Jan 31 2006

Ab8162 is useful for inhibition assays - inhibition of the biological activity of TNF-alpha. However as far as we know this application is not mentioned in any article, yet and we don't have a specific protocol available.

As far as we are aware, ab8162 has not yet been tested in IHC.