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Recombinant full length protein (Human).
This antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 µg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells.
Our Abpromise guarantee covers the use of ab9809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|Indirect ELISA||1/1000 - 1/10000.|
|Neutralising||Use at an assay dependent concentration.|
|WB||1/1000 - 1/5000.|