製品の概要
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製品名Anti-TNF alpha antibody [MP6-XT3]
TNF alpha 一次抗体 製品一覧 -
製品の詳細Rat Monoclonal [MP6-XT3] to TNF alpha
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特異性ab11564 recognises murine TNF alpha. Reactivity with murine TNF Beta has not yet been determined.
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アプリケーション適用あり: WB, Flow Cyt, ELISAmore details
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種交差性交差種: Mouse, Rat
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免疫原
Recombinant full length protein corresponding to Mouse TNF alpha. Expressed in E. coli.
Database link: P06804 -
特記事項This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
精製度Protein G purified
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特記事項（精製）Purified IgG prepared by affinity chromatography from tissue culture supernatant.
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ポリ/モノモノクローナル
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クローン名MP6-XT3
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アイソタイプIgG1
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研究分野
関連製品
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Affibody® Molecule
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab11564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. This antibody recognises the 17 kDa soluble form of TNF alpha. | |
| Flow Cyt | Use at an assay dependent concentration. Membrane permeabilization is required for this application ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ELISA | Use a concentration of 5 µg/ml. |
ターゲット情報
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機能Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
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関連疾患Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
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配列類似性Belongs to the tumor necrosis factor family.
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翻訳後修飾The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
細胞内局在Secreted and Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 21926 Mouse
- Entrez Gene: 24835 Rat
- SwissProt: P06804 Mouse
- SwissProt: P16599 Rat
- Unigene: 1293 Mouse
- Unigene: 2275 Rat
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別名
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
Anti-TNF alpha antibody [MP6-XT3] 画像
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![Sandwich ELISA - Anti-TNF alpha antibody [MP6-XT3 ] (ab11564)](http://a.abcam.com/ps/datasheet/images/11/ab11564/TNF-alpha-Primary-antibodies-ab11564-1.jpg)
Sandwich ELISA - Anti-TNF alpha antibody [MP6-XT3 ] (ab11564)Recombinant Mouse TNF alpha detected in a sandwich assay with Rat anti Mouse TNF alpha (ab11564) as the coating antibody; Rabbit anti Mouse TNF alpha:Biotin as the detetction antibody and Streptavidin:HRP as the final layer.
Anti-TNF alpha antibody [MP6-XT3] (ab11564) 使用論文
This product has been referenced in:
- Patki G et al. Tempol treatment reduces anxiety-like behaviors induced by multiple anxiogenic drugs in rats. PLoS One 10:e0117498 (2015). WB ; Rat . Read more (PubMed: 25793256) »
- Sousa R et al. Effect of Lactobacillus acidophilus supernatants on body weight and leptin expression in rats. BMC Complement Altern Med 8:5 (2008). Rat . Read more (PubMed: 18284678) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"