Anti-TNF alpha 抗体 [MP6-XT3] (ab11564)
Key features and details
- Rat monoclonal [MP6-XT3] to TNF alpha
- Suitable for: ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-TNF alpha antibody [MP6-XT3]
TNF alpha 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [MP6-XT3] to TNF alpha -
由来種
Rat -
特異性
ab11564 recognises murine TNF alpha. Reactivity with murine TNF Beta has not yet been determined. -
アプリケーション
適用あり: ELISAmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Mouse TNF alpha. Expressed in E. coli.
Database link: P06804 -
特記事項
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified IgG prepared by affinity chromatography from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
MP6-XT3 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Affibody® Molecule
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11564の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use a concentration of 5 µg/ml.
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特記事項 |
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ELISA
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
関連疾患
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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別名
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (9)
ab11564 は 9 報の論文で使用されています。
- Xu Y et al. Identification of the Key Role of NF-?B Signaling Pathway in the Treatment of Osteoarthritis With Bushen Zhuangjin Decoction, a Verification Based on Network Pharmacology Approach. Front Pharmacol 12:637273 (2021). PubMed: 33912052
- Lin Z et al. Improving Small Intestinal Motility in Experimental Acute Necrotising Pancreatitis by Modulating the CPI-17/MLCP Pathway Using Chaiqin Chengqi Decoction. Evid Based Complement Alternat Med 2020:9189457 (2020). PubMed: 32104199
- Chen C et al. Transcriptomic study of lipopolysaccharide-induced sepsis damage in a mouse heart model. Exp Ther Med 20:3782-3790 (2020). PubMed: 32855727
- Yu B et al. Morroniside on anti-inflammation activities in rats following acute myocardial infarction. Korean J Physiol Pharmacol 22:17-21 (2018). PubMed: 29302208
- Lin JR et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). PubMed: 29993362
- Peng L et al. Lentivirus-mediated TNF-a gene silencing and overexpression of osteoprotegerin inhibit titanium particle-induced inflammatory response and osteoclastogenesis in vitro. Mol Med Rep 13:1010-8 (2016). PubMed: 26648171
- Patki G et al. Tempol treatment reduces anxiety-like behaviors induced by multiple anxiogenic drugs in rats. PLoS One 10:e0117498 (2015). WB ; Rat . PubMed: 25793256
- Wang CP et al. Mulberroside A protects against ischemic impairment in primary culture of rat cortical neurons after oxygen-glucose deprivation followed by reperfusion. J Neurosci Res 92:944-54 (2014). PubMed: 24687774
- Sousa R et al. Effect of Lactobacillus acidophilus supernatants on body weight and leptin expression in rats. BMC Complement Altern Med 8:5 (2008). Rat . PubMed: 18284678