製品の概要

  • 製品名
    Anti-TNF alpha antibody [MP6-XT3]
    TNF alpha 一次抗体 製品一覧
  • 製品の詳細
    Rat Monoclonal [MP6-XT3] to TNF alpha
  • 由来種
    Rat
  • 特異性
    ab11564 recognises murine TNF alpha. Reactivity with murine TNF Beta has not yet been determined.
  • アプリケーション
    適用あり: WB, Flow Cyt, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Recombinant full length protein corresponding to Mouse TNF alpha. Expressed in E. coli.
    Database link: P06804

  • 特記事項
    This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. This antibody recognises the 17 kDa soluble form of TNF alpha.
Flow Cyt Use at an assay dependent concentration.

Membrane permeabilization is required for this application

 

 

 

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ELISA Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 関連疾患
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 配列類似性
    Belongs to the tumor necrosis factor family.
  • 翻訳後修飾
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • APC1 antibody
      • APC1 protein antibody
      • Cachectin antibody
      • DIF antibody
      • Differentiation inducing factor antibody
      • Macrophage cytotoxic factor antibody
      • Tnf antibody
      • TNF superfamily, member 2 antibody
      • TNF, macrophage derived antibody
      • TNF, monocyte derived antibody
      • TNF-a antibody
      • TNF-alpha antibody
      • TNFA antibody
      • TNFA_HUMAN antibody
      • TNFSF2 antibody
      • Tumor necrosis factor alpha antibody
      • Tumor necrosis factor antibody
      • Tumor necrosis factor ligand superfamily member 2 antibody
      • Tumor Necrosis Factor, Membrane Form antibody
      • Tumor necrosis factor, soluble form antibody
      see all

    画像

    • Sandwich ELISA - Anti-TNF alpha antibody [MP6-XT3] (ab11564)
      Sandwich ELISA - Anti-TNF alpha antibody [MP6-XT3] (ab11564)
      Recombinant Mouse TNF alpha detected in a sandwich assay with Rat anti Mouse TNF alpha (ab11564) as the coating antibody; Rabbit anti Mouse TNF alpha:Biotin as the detetction antibody and Streptavidin:HRP as the final layer.

    参考文献

    This product has been referenced in:
    • Yu B  et al. Morroniside on anti-inflammation activities in rats following acute myocardial infarction. Korean J Physiol Pharmacol 22:17-21 (2018). Read more (PubMed: 29302208) »
    • Patki G  et al. Tempol treatment reduces anxiety-like behaviors induced by multiple anxiogenic drugs in rats. PLoS One 10:e0117498 (2015). WB ; Rat . Read more (PubMed: 25793256) »

    See all 3 Publications for this product

    ab11564 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    4 reviews or Q&A

    10
    Votes: Highest First

    Hello, We are developping a double elispot IFNg/TNFa in mouse. We already have an IFNg elispot with biotin / streptavidin / HRP detection system, so we are looking for antibodies for the TNFa part. I have seen these antibod...

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    Thank you very much for your inquiry. Reviewing the datasheets for these antibody I am confident that a single ELISPOT should give good results with this combination of antibodies. But unfortunately, these antibodies have not been tested in...

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    Thanks for your response. So what is this integral membrane protein? Actually, the band I am getting is close to 25 kDa. So this is not TNF-alpha? Please tell me more about this integral membrane protein. I thought it is TNF-alpha because it is really ...

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    Thank you for your email. Our source for this antibody tested ab11564 in Western blotting with recombinant TNF alpha. Conditions were standard. To use this antibody in Western blotting, I would recommend referring to our general Western blot protocol w...

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    What size band should be seen with this antibody? 28 kDa or 17 kDa?

    This antibody recognises the 17 kDa soluble form of TNF alpha. TNF alpha forms dimers and trimers, and in addition is found as a 26 kDa integral membrane protein. I am not able to comment on the ability of this antibody to recognise the membrane bound ...

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    I am interested in purchasing your TNF alpha antibodies, ab11564 and ab11546. The product datasheets contain the statement, "Reactivity with murine TNF Beta has not yet been determined". Can you tell me whether this information is up-to-date please? Al...

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    Thank you for your enquiry and for your patience. All the information that we have regarding these two antibodies is loacted on the online datasheets. To our knowledge, reactivity with TNF beta has still not yet been tested. Mature TNF-beta and TNF-alp...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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