製品の概要

  • 製品名Anti-TNF alpha antibody
    TNF alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TNF alpha
  • アプリケーション適用あり: IHC-Fr, IHC-P, WB, ELISA, Neutralising, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment corresponding to Mouse TNF alpha aa 80-235. E.coli-derived recombinant MurineTNF-alpha
    Database link: P06804

  • ポジティブ・コントロール
    • This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A431

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 18458097
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. To detect mTNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mTNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. The presursor is ~26 kDa and the secreted form is ~17 kDa.
ELISA Use at an assay dependent concentration.

To detect Murine TNF-alpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF-alpha.

Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of mTNF-alpha (0.25 ng/ml), a concentration of 0.08 – 0.10 µg/ml of this antibody is required.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 関連疾患Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 配列類似性Belongs to the tumor necrosis factor family.
  • 翻訳後修飾The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

Anti-TNF alpha antibody 画像

  • All lanes : Anti-TNF alpha antibody (ab9739) at 1/3500 dilution

    Lane 1 : 100ug LPS stimulated J774.A1 mice macrophages
    Lane 2 : 50ug LPS stimulated J774.A1 mice macrophages
    Lane 3 : 25ug LPS stimulated J774.A1 mice macrophages

    Secondary
    HRP conjugated goat anti-rabbit antibody

    Performed under reducing conditions.

    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Dr sandra sobocanec

    See Abreview

  • ab9739 (2µg/ml) staining TNF alpha in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining within the germinal follicle.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab9739 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9739 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TNF alpha antibody (ab9739) 使用論文

This product has been referenced in:
  • Moghimi E  et al. Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis. PLoS One 10:e0126355 (2015). WB ; Mouse . Read more (PubMed: 26020962) »
  • Marino FE  et al. Activin-ßC modulates cachexia by repressing the ubiquitin-proteasome and autophagic degradation pathways. J Cachexia Sarcopenia Muscle 6:365-80 (2015). WB ; Mouse . Read more (PubMed: 26673867) »

See all 18 Publications for this product

Product Wall

Application Western blot
Sample Mouse Serum (Mouse Serum)
Gel Running Conditions Reduced Non-Denaturing (Native)
Loading amount 20 µg
Specification Mouse Serum
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
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投稿 Jul 30 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (hepatocytes)
Specification hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Jul 08 2014

Thank you for contacting us.

Unfortunately, we cannot test every organ or tissue when we establish a product.However, if the knee joints were decalcified properly and the species is tested, I don't see any problems why these antibodies shoul...

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All of these antibodies recognise cytokines which are present in inflammed tissue therefore any inflammed tissue would be a good positive control.

In our catalogue, we have several cancer slides which would be appropriate:
tonsil: (ab514...

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Vielen Dank für Ihre Anfrage.

Ich habe vier primäre AK für Sie herausgesucht, die alle in einer relativ hohen Verdünnung eingesetzt werden können, so dass sie für eine Weile reichen sollten. Das Sie alle Proben nu...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unserem Wissen nach wurde ab17442 bisher noch nicht in sandwichELISA getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zurzeit ein spezielles...

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Thank you for contacting us and for your interest in our products.

As far as we are aware, ab9739 has never been tested in IP. All tested applications covered by the 6 month guarantee are specified on our datasheets, and these are updated as...

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Thank you very much for your reply.

You are correct, and I apologize for this mistake. I converted the 100 micrograms into milligrams, not grams as it should have been for the calculation. There should be 4.01e14 molecules per vial. Thank you...

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Thank you very much for your reply.

There are approximately 4.01 x 10^17 molecules of anti-TNF alpha IgG per 100microgram vial. This is calculated as:

Molecular weight of IgG= 150 kDa= 150,000 g/mol

100 micrograms divided ...

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Thank you very much for your call and for your enquiry.

Ab9739 was raised against the recombinant soluble form of murine TNF alpha. As this is a polyclonal antibody and the soluble form shares homology with the membrane form, it should bind t...

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1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"