製品の概要

  • 製品名Anti-TMPRSS2 antibody [EPR3861]
    TMPRSS2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3861] to TMPRSS2
  • アプリケーション適用あり: WB, IHC-P, ICC, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Rat, Human, Cynomolgus Monkey, Rhesus monkey, Marmoset (common)
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TMPRSS2 aa 1-100.

  • ポジティブ・コントロール
    • Human prostate, Human small intestine and LNCaP cell lysates, Human prostatic adenocarcinoma
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:
    Anti-TMPRSS2 antibody (HRP) [EPR3861] (ab199095)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
IHC-P 1/1000.

The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these.

ICC 1/250 - 1/1000.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 組織特異性Expressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland.
    • 配列類似性Belongs to the peptidase S1 family.
      Contains 1 LDL-receptor class A domain.
      Contains 1 peptidase S1 domain.
      Contains 1 SRCR domain.
    • 細胞内局在Cell membrane and Secreted. Activated by cleavage and secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • D16Ertd61e antibody
      • Epitheliasin antibody
      • FLJ41954 antibody
      • MGC6821 antibody
      • PP9284 antibody
      • PRSS10 antibody
      • Serine protease 10 antibody
      • TMPRSS2 antibody
      • TMPRSS2 ERG FUSION GENE, INCLUDED antibody
      • TMPRSS2 ETV1 FUSION GENE, INCLUDED antibody
      • TMPS2_HUMAN antibody
      • Transmembrane protease serine 2 catalytic chain antibody
      • Transmembrane protease, serine 2 antibody
      • Transmembrane protease, serine 2, EC 3.4.219 antibody
      see all

    Anti-TMPRSS2 antibody [EPR3861] 画像

    • All lanes : Anti-TMPRSS2 antibody [EPR3861] (ab92323) at 1/1000 dilution

      Lane 1 : Human prostate lysate
      Lane 2 : Human small intestine lysate
      Lane 3 : LNCaP cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size : 54 kDa
    • ab92323 at 1/1000 dilution, staining TMPRSS2 in paraffin embedded Human prostatic adenocarcinoma
    • Overlay histogram showing LoVo cells stained with ab92323 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92323, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    Anti-TMPRSS2 antibody [EPR3861] (ab92323) 使用論文

    This product has been referenced in:
    • Esumi M  et al. Transmembrane serine protease TMPRSS2 activates hepatitis C virus infection. Hepatology 61:438-47 (2015). Read more (PubMed: 25203900) »
    • Chu M  et al. Hypermethylation-mediated transcriptional repression of TMPRSS2 in androgen receptor-negative prostate cancer cells. Exp Biol Med (Maywood) N/A:N/A (2014). Read more (PubMed: 24764242) »

    See all 2 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (DU145 Prostate cancer cell line)
    Permeabilization Yes - 0.1% v/v TritonX-100
    Specification DU145 Prostate cancer cell line
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Dr. Dimitra Kalamida

    Verified customer

    投稿 Jun 06 2016

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (12,5%)
    Sample Rhesus monkey Cell lysate - whole cell (293T HEK transfected with rhesus TMPRSS2)
    Specification 293T HEK transfected with rhesus TMPRSS2
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 26 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"