製品の概要

  • 製品名
    Anti-TMIE antibody
  • 製品の詳細
    Rabbit polyclonal to TMIE
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 78-108 of Human TMIE

  • ポジティブ・コントロール
    • Mouse liver nuclear lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab103028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB 1/100 - 1/500. Predicted molecular weight: 17 kDa.

ターゲット情報

  • 関連性
    The TMIE gene encodes a transmembrane inner ear protein. TMIE is required for normal postnatal maturation of sensory hair cells in the cochlea, including correct development of stereocilia bundles, in mice. It is one of multiple genes responsible for recessive non-syndromic deafness (DFNB). This is also known as autosomal recessive nonsyndromic hearing loss (ARNSHL), the most common form of congenitally acquired inherited hearing impairment.
  • 細胞内局在
    Membrane; Single-pass type I membrane protein
  • 参照データベース
  • 別名
    • deafness autosomal recessive 6 antibody
    • DFNB6 antibody
    • transmembrane inner ear antibody
    • transmembrane inner ear expressed protein antibody
    • transmembrane inner ear protein antibody
    see all

Anti-TMIE antibody 画像

  • ab103028 staining TMIE in mouse COS-7 cells transfected with TMIE-cherry plasmid by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 4% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/400) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Anti-TMIE antibody (ab103028) at 1/100 dilution + Mouse liver tissue lysate at 12.5 µg

    Predicted band size : 17 kDa

Anti-TMIE antibody (ab103028) 使用論文

ab103028 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (COS-7 cells)
Specification
COS-7 cells
Permeabilization
Yes - Triton X-100
Fixative
Paraformaldehyde
Username

Dr. Angela Ballesteros Morcillo

Verified customer

投稿 May 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録