Rabbit polyclonal to TMEM74
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
Synthetic peptide corresponding to a region within the internal sequence amino acids 216-265 (RLEKESARLG AHLDRCVIAG LCLLTLGGVI LSCLLMMSMW KGELYRRNRF) of human TMEM74 (NP_694560).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Purified by peptide affinity chromatography method.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
TMEM74 plays an essential role in autophagy. TMEM74-induced autophagy may involve PI3K signal transduction. It is expressed in heart, lung and placenta.
Lysosome membrane; Multi pass membrane protein. Cytoplasmic vesicle, autophagosome membrane; Multi pass membrane protein.
- Transmembrane protein 74 antibody
Western blot - TMEM74 antibody (ab87239)
Anti-TMEM74 antibody (ab87239) at 1 µg/ml + human fetal heart lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
33 kDaObserved band size:
37 kDa (why is the actual band size different from the predicted?
)Additional bands at:
20 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"