Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
Purified by peptide affinity chromatography method.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
TMEM74 plays an essential role in autophagy. TMEM74-induced autophagy may involve PI3K signal transduction. It is expressed in heart, lung and placenta.
Lysosome membrane; Multi pass membrane protein. Cytoplasmic vesicle, autophagosome membrane; Multi pass membrane protein.
- Transmembrane protein 74 antibody
Anti-TMEM74 antibody 画像
Western blot - TMEM74 antibody (ab87239)
Anti-TMEM74 antibody (ab87239) at 1 µg/ml + human fetal heart lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
33 kDaObserved band size :
37 kDa (why is the actual band size different from the predicted?
)Additional bands at :
20 kDa. We are unsure as to the identity of these extra bands.
Anti-TMEM74 antibody (ab87239) 使用論文
has not yet been referenced specifically in any publications.
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