Anti-TMEM25 抗体 (ab121362)
Key features and details
- Rabbit polyclonal to TMEM25
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TMEM25 antibody -
製品の詳細
Rabbit polyclonal to TMEM25 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
antigen, corresponding to amino acids 262-364 of Human TMEM25 (Uniprot: Q86YD3)
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ポジティブ・コントロール
- Human duodenum tissue and Liver lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121362の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/250 - 1/500.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/250 - 1/500. |
ターゲット情報
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配列類似性
Contains 1 Ig-like (immunoglobulin-like) domain. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 84866 Human
- Omim: 613934 Human
- SwissProt: Q86YD3 Human
- Unigene: 564188 Human
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別名
- 0610039J01Rik antibody
- FLJ14399 antibody
- Tmem25 antibody
see all
画像
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Immunohistochemical analysis of human duodenum tissue labelled for TMEM25 using ab121362 at a dilution of 1/50. Positive cytoplasmic staining in glandular cells.
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Immunohistochemical analysis of human cerebellum tissue labelled for TMEM25 using ab121362 at a dilution of 1/50. Positive cytoplasmic staining in granular layer cells.
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Immunohistochemical analysis of human kidney tissue labelled for TMEM25 using ab121362 at a dilution of 1/50. Positive cytoplasmic staining in human tubule cells.
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Immunohistochemical analysis of human prostate tissue labelled for TMEM25 using ab121362 at a dilution of 1/50. Positive cytoplasmic and membranous staining in human glandular cells.
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All lanes : Anti-TMEM25 antibody (ab121362) at 1/250 dilution
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human Plasma lysate
Lane 4 : Liver lysate
Lane 5 : Tonsil lysate -
Immunofluorescent staining of human cell line A-431 shows positivity in vesicles.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab121362 は論文での使用が確認できていません。