製品の概要

  • 製品名
    Anti-TMEM180 antibody
  • 製品の詳細
    Rabbit polyclonal to TMEM180
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 361-411 (CLFIASNRVF TEGTCKLLTL VVTDLVDEDL VLNHRKQAAS ALLFGMVALV T) of Human TMEM180 (NP_079065).

  • ポジティブ・コントロール
    • THP-1 cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-TMEM180 antibody 画像

  • Anti-TMEM180 antibody (ab108080) at 1 µg/ml + THP-1 cell lysate at 10 µg

    Predicted band size : 57 kDa

    Gel concentration: 12%

     

    Secondary antibody - anti-rabbit HRP (ab6721)

Anti-TMEM180 antibody (ab108080) 使用論文

ab108080 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Skin Fibroblasts)
Loading amount
40 µg
Specification
Skin Fibroblasts
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 May 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Skin fibroblasts)
Specification
Skin fibroblasts
Fixative
Methanol
Permeabilization
Yes - 0.1% triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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