Anti-TMEM16B 抗体 (ab113443)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TMEM16B
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide from the N terminal region of Human TMEM16B, conjugated to KLH.

  • ポジティブ・コントロール
    • Human small intestine and spleen tissues; Rat brain tissue lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 114 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.
  • 組織特異性
    Retina, especially in the photoreceptor synaptic terminals.
  • 配列類似性
    Belongs to the anoctamin family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ano2 antibody
    • ANO2_HUMAN antibody
    • Anoctamin 2 antibody
    • Anoctamin-2 antibody
    • C12orf3 antibody
    • Chromosome 12 open reading frame 3 antibody
    • DKFZp434P102 antibody
    • Transmembrane protein 16B (eight membrane-spanning domains) antibody
    • Transmembrane protein 16B antibody
    see all

画像

  • ab113443, at 5 µg/ml, staining TMEM16B in formalin fixed, paraffin embedded Human small intestine tissue by Immunohistochemistry.
  • ab113443, at 5 µg/ml, staining TMEM16B in formalin fixed, paraffin embedded Human spleen tissue by Immunohistochemistry.
  • Lane 1 : Anti-TMEM16B antibody (ab113443) at 1 µg/ml
    Lane 2 : Anti-TMEM16B antibody (ab113443) at 2 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate


    Predicted band size : 114 kDa

参考文献

ab113443 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: PBS/HCl/Borax
Permeabilization
Yes - 0.2% PBS-Tx
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 26°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 07 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon Ca)
Specification
Colon Ca
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 18 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (retina)
Specification
retina
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Zytomed Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0% · Temperature: 20°C
Username

Ms. Andrea Dannullis

Verified customer

投稿 Oct 17 2012

The laboratory that produces ab113443 has sent the alignment of the immunogen with the rat and mouse proteins. As I mentioned in my previous e-mail, the identity of the immunogen sequence is considered proprietary. Homology between human and mo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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