製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TMEM16A
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 828-877 (HGFVNHTLSS FNVSDFQNGT APNDPLDLGY EVQICRYKDY REPPWSENKY) of Human TMEM16A (NP_060513).

  • ポジティブ・コントロール
    • Human fetal muscle lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 114 kDa.Can be blocked with TMEM16A peptide (ab207564). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Acts as a calcium-activated chloride channel. Required for normal tracheal development.
  • 組織特異性
    Broadly expressed with higher levels in liver and skeletal muscle.
  • 配列類似性
    Belongs to the anoctamin family.
  • ドメイン
    The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
  • 細胞内局在
    Cell membrane. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANO 1 antibody
    • ANO1 antibody
    • ANO1_HUMAN antibody
    • Anoctamin 1 antibody
    • Anoctamin 1 calcium activated chloride channel antibody
    • Anoctamin-1 antibody
    • Anoctamin1 antibody
    • Ca2+ activated Cl- channel antibody
    • Calcium Activated Chloride Channel antibody
    • Discovered on gastrointestinal stromal tumors protein 1 antibody
    • DOG 1 antibody
    • DOG1 antibody
    • FLJ10261 antibody
    • Membrane protein antibody
    • Oral cancer overexpressed 2 antibody
    • Oral cancer overexpressed protein 2 antibody
    • ORAOV 2 antibody
    • ORAOV2 antibody
    • TAOS 2 antibody
    • TAOS2 antibody
    • TMEM 16A antibody
    • TMEM16A antibody
    • Transmembrane protein 16A (eight membrane spanning domains) antibody
    • Transmembrane protein 16A antibody
    • Tumor amplified and overexpressed sequence 2 antibody
    • Tumor-amplified and overexpressed sequence 2 antibody
    see all

画像

  • Anti-TMEM16A antibody (ab84115) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 114 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Crutzen R  et al. Anoctamin 1 (Ano1) is required for glucose-induced membrane potential oscillations and insulin secretion by murine ß-cells. Pflugers Arch 468:573-91 (2016). Read more (PubMed: 26582426) »

See 1 Publication for this product

レビューと Q&A

I have had the opportunity to review our anti-TMEM16A antibodies and have found two which will recognize the extracelluar domain of this target. http://www.abcam.com/tmem16a-antibody-ab84115.html was created from a peptide corresponding to a region...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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