Anti-TMEM16A 抗体 (ab72984)
Key features and details
- Rabbit polyclonal to TMEM16A
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TMEM16A antibody
TMEM16A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TMEM16A -
由来種
Rabbit -
特異性
This antibody reacts specifically with human 114 kDa TMEM16A protein. -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human TMEM16A aa 700-800 (internal sequence).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72984の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/1000.
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特記事項 |
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ICC/IF
1/1000. |
ターゲット情報
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機能
Acts as a calcium-activated chloride channel. Required for normal tracheal development. -
組織特異性
Broadly expressed with higher levels in liver and skeletal muscle. -
配列類似性
Belongs to the anoctamin family. -
ドメイン
The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region. -
細胞内局在
Cell membrane. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 55107 Human
- Omim: 610108 Human
- SwissProt: Q5XXA6 Human
- Unigene: 503074 Human
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別名
- ANO 1 antibody
- ANO1 antibody
- ANO1_HUMAN antibody
see all
画像
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ICC/IF image of ab72984 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72984, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
データシートおよび資料
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Datasheet download
参考文献 (9)
ab72984 は 9 報の論文で使用されています。
- Heng J et al. miR-106a Targets Anoctamin 1 (ANO1) to Regulate Lipopolysaccharide (LPS)-Induced Inflammatory Response in Macrophages. Med Sci Monit 26:e922479 (2020). PubMed: 33037174
- Chen D et al. ANO1 in urethral SMCs contributes to sex differences in urethral spontaneous tone. Am J Physiol Renal Physiol 319:F394-F402 (2020). PubMed: 32686521
- Tong JJ et al. Calcium-Activated Chloride Channels in Newly Differentiating Mouse Lens Fiber Cells and Their Role in Volume Regulation. Invest Ophthalmol Vis Sci 60:1621-1629 (2019). PubMed: 30995319
- Banerjee J et al. Regulatory Roles of Anoctamin-6 in Human Trabecular Meshwork Cells. Invest Ophthalmol Vis Sci 58:492-501 (2017). WB . PubMed: 28125837
- Crutzen R et al. Anoctamin 1 (Ano1) is required for glucose-induced membrane potential oscillations and insulin secretion by murine ß-cells. Pflugers Arch 468:573-91 (2016). PubMed: 26582426
- Davis AJ et al. Potent vasorelaxant activity of the TMEM16A inhibitor T16A(inh) -A01. Br J Pharmacol 168:773-84 (2013). PubMed: 22946562
- Wang M et al. Downregulation of TMEM16A calcium-activated chloride channel contributes to cerebrovascular remodeling during hypertension by promoting basilar smooth muscle cell proliferation. Circulation 125:697-707 (2012). WB ; Rat . PubMed: 22215857
- Hanzu FA et al. Expression of TMEM16A and SLC4A4 in human pancreatic islets. Cell Physiol Biochem 29:61-4 (2012). PubMed: 22415075
- Davis AJ et al. Expression profile and protein translation of TMEM16A in murine smooth muscle. Am J Physiol Cell Physiol 299:C948-59 (2010). IHC-Fr ; Mouse . PubMed: 20686072