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Our Abpromise guarantee covers the use of ab53212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2 µg/ml. PubMed: 19819874|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21084687|
Immunocytochemistry/ Immunofluorescence analysis of Wt1cre-YFP mouse interstitial cell of Cajal labelling TMEM16A with ab53212 at 1/20 dilution. Cells from the muscular layer, especially the circular one, show TMEM16A (ANO1 - red) immunoreactivity. This immunostaining becomes strong by E16.5. Colocalization of YFP with ANO1 is observed in many cells of both, the circular and the longitudinal layer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastrointestinal stromal tumor labeling TMEM16A with ab53212.