製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TMEM16A
  • アプリケーション
    適用あり: IHC-P, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide of human TMEM16A protein

  • ポジティブ・コントロール
    • GIST

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100.
ICC/IF Use a concentration of 2 µg/ml. PubMed: 19819874
IHC-Fr Use at an assay dependent concentration. PubMed: 21084687

ターゲット情報

  • 機能
    Acts as a calcium-activated chloride channel. Required for normal tracheal development.
  • 組織特異性
    Broadly expressed with higher levels in liver and skeletal muscle.
  • 配列類似性
    Belongs to the anoctamin family.
  • ドメイン
    The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
  • 細胞内局在
    Cell membrane. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANO 1 antibody
    • ANO1 antibody
    • ANO1_HUMAN antibody
    • Anoctamin 1 antibody
    • Anoctamin 1 calcium activated chloride channel antibody
    • Anoctamin-1 antibody
    • Anoctamin1 antibody
    • Ca2+ activated Cl- channel antibody
    • Calcium Activated Chloride Channel antibody
    • Discovered on gastrointestinal stromal tumors protein 1 antibody
    • DOG 1 antibody
    • DOG1 antibody
    • FLJ10261 antibody
    • Membrane protein antibody
    • Oral cancer overexpressed 2 antibody
    • Oral cancer overexpressed protein 2 antibody
    • ORAOV 2 antibody
    • ORAOV2 antibody
    • TAOS 2 antibody
    • TAOS2 antibody
    • TMEM 16A antibody
    • TMEM16A antibody
    • Transmembrane protein 16A (eight membrane spanning domains) antibody
    • Transmembrane protein 16A antibody
    • Tumor amplified and overexpressed sequence 2 antibody
    • Tumor-amplified and overexpressed sequence 2 antibody
    see all

Anti-TMEM16A antibody 画像

  • Immunocytochemistry/ Immunofluorescence analysis of Wt1cre-YFP mouse interstitial cell of Cajal labelling TMEM16A with ab53212 at 1/20 dilution. Cells from the muscular layer, especially the circular one, show TMEM16A (ANO1 - red) immunoreactivity. This immunostaining becomes strong by E16.5. Colocalization of YFP with ANO1 is observed in many cells of both, the circular and the longitudinal layer.

  • ab53212 (2µg/ml) staining TMEM16A in human liver (left panel) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of hepatocyte cell membrane.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Analysis of TMEM16A expression was performed in frozen liver sections (10 µm) from normal and 7-day bile duct-ligated rats and mice.
    Sections were defrosted at room temperature and fixed in 4% paraformaldehyde (1× PBS), permeabilized in PBST (1× PBS with 0.2% Triton), and blocked in 4% BSA (in PBST) for 1 hour at room temperature. Samples were then incubated with ab53212 at a 1/100 dilution, diluted in 1% BSA. Sections were incubated overnight at 4 °C and washed three times for 10 minutes each with 1× PBST at room temperature. Sections were incubated with Dylight 488 conjugated donkey anti-rabbit secondary at a 1/600 dilution for 2 hours at room temperature protected from light. Following incubation, the slides were washed in PBST at room temperature and coverslipped with Antifade gold and DAPI. Images were visualized using an Olympus IX-71 confocal microscope.

    Scale bar, 10 µm.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastrointestinal stromal tumor labeling TMEM16A with ab53212.

  • ICC/IF image of ab53212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53212, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TMEM16A antibody (ab53212) 使用論文

This product has been referenced in:
  • Zaidman NA  et al. Large-conductance Ca(2+) -activated K(+) channel activation by apical P2Y receptor agonists requires hydrocortisone in differentiated airway epithelium. J Physiol 595:4631-4645 (2017). Human . Read more (PubMed: 28481415) »
  • Kamikawa A  et al. Ca2+-activated Cl- channel currents in mammary secretory cells from lactating mouse. Am J Physiol Cell Physiol 311:C808-C819 (2016). Read more (PubMed: 27558156) »

See all 13 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 11 2016

ab53213 is the prediluted version of ab53212, otherwise they are the same product.

Thank you for your reply and for kindly providing the word document. This has made conducting the alignments much more straight forward.

I am sorry to confirm that as far as we are aware,none of our TMEM16A antibodieshas been tested with sam...

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Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

Unfortunately we are not able to provide the blocking peptide for ab53212 at this time. I am very sorry that this product ...

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Thank you for your inquiry.

I can confirm that the IHC-Fr image was not generated by our lab but is taken from the following publication:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3013035/?tool=pubmed

Please follow the a...

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Thank you for contacting us.
This image is from publication; Dutta AK et al, J Biol Chem. 2011 Jan 7;286(1):766-76. Epub 2010 Nov 1, Fig 1. DOI 10.1074/jbc.M110.164970. Customer can ask this to Author of the paper.
We also have DAPI mounting m...

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Thanks for your reply.

To calculate the 89% score, did you use the canis lupus sequence that I sent or a different dog sequence? I ran the BLAST again and the Identity score came up as 94%, so I'm not sure if we're looking at the same canine ...

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Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Hu...

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Thank you for contacting us. Unfortunately, we do not currently have a blocking peptide for ab53212 available for purchase. The immunogen for this antibody is located in the range between amino acids 100- 450 of human TMEM16A. You may be able ...

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Thank you very much for filling the questionnaire. We have added rat as species from a publication Pub med ID 21041307. Unfortunately we do not have our own lab tested data about this species. The predicted band size for this protein is 114kDa;...

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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