製品の概要

  • 製品名
    Anti-TMEM146 antibody
  • 製品の詳細
    Rabbit polyclonal to TMEM146
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within residues: LIQDVQGDRL YFHPTTTRLI KHPCEKNIAL YLGKQVFFTM DNFETSLLPF , corresponding to N terminal amino acids 36-85 of Human TMEM146 (NP_689997)

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

ターゲット情報

  • 関連性
    TMEM146 is a single-pass type I membrane protein. The functions of TMEM146 remain unknown.
  • 細胞内局在
    Membrane; Single-pass type I membrane protein
  • 参照データベース
  • 別名
    • 4933402B14Rik antibody
    • AW045609 antibody
    • MGC189565 antibody
    • MGC39581 antibody
    • rCG_45086 antibody
    • Transmembrane protein 146 antibody
    see all

画像

  • Anti-TMEM146 antibody (ab83738) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 90 kDa
    Observed band size : 90 kDa
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab83738 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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