Anti-TMEM132D 抗体 (ab116041)

製品の概要

  • 製品名
    Anti-TMEM132D antibody
  • 製品の詳細
    Rabbit polyclonal to TMEM132D
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 700-749(

    QRPKQEAAISCWVQFSDGSVTPLDIYDEKDFSLMATSLDEKVVSILQDPK

    ) of Mouse TMEM132D (NP_766473).

  • ポジティブ・コントロール
    • Mouse liver lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Constituents: 2% Sucrose, 97% PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab116041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 122 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Anti-TMEM132D antibody (ab116041) at 1 µg/ml + mouse liver lysate at 10 µg

    Predicted band size : 122 kDa

プロトコール

参考文献

ab116041 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
The details you have kindly provided will enable us to investigate this case ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (INS1 cells)
Loading amount
30000 cells
Specification
INS1 cells
Gel Running Conditions
Reduced Denaturing (8 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Olatz Villate

Verified customer

投稿 Mar 15 2012

Thank you for contacting us.
Ab116041 has never been tested in rat. It doesn’t mean it won’t work in this species, but we don’t have data to support it. I performed a BLAST to evaluate the percentage of homology with rat species, and it’s very lik...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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