Anti-TMEM120B 抗体 (ab121413)
Key features and details
- Rabbit polyclonal to TMEM120B
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TMEM120B antibody -
製品の詳細
Rabbit polyclonal to TMEM120B -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
antigen: THRIYKQKLE ELAALQTLCS SSISKQKKHL KDLKLTLQRC KRHASREEAE LVQQMAANIK ERQDVFFDME AYLPKKNGLY LNLVLGNVNV TLLSNQAKFA YKD, corresponding to amino acids 25-127 of Human TMEM120B (A0PK00).
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ポジティブ・コントロール
- Human stomach tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121413の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
1/100 - 1/250. Predicted molecular weight: 40 kDa.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
1/100 - 1/250. Predicted molecular weight: 40 kDa. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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配列類似性
Belongs to the TMEM120 family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 144404 Human
- SwissProt: A0PK00 Human
- Unigene: 644504 Human
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別名
- T120B_HUMAN antibody
- TMEM120B antibody
- Transmembrane protein 120B antibody
画像
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Lane 1 : Anti-TMEM120B antibody (ab121413) at 1/100 dilution
Lane 2 : Anti-TMEM120B antibody (ab121413)
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag (~3.1kDa) in HEK293T cells.
Predicted band size: 40 kDa -
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleoli and cytoplasm. Recommended concentration of ab121413 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemical staining of Human stomach tissue shows strong cytoplasmic positivity in glandular cells, using 1/20 ab121413.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab121413 は論文での使用が確認できていません。