The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Predicted molecular weight: 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Titre using peptide based assay 1/312500.
TLX2 contains 1 homeobox DNA-binding domain and the function remains unknown.
In the mouse, this gene is expressed from embryonic day 9.5 through day 13.5 and is detectable in the dorsal root ganglia, cranial and enteric nerve ganglia and adrenal glands. In adult animals its expression is limited to the adrenal gland and the intestine. TLX2 may be involved in the proliferation and/or differentiation of enteric peripheral nervous system.