製品の概要

  • 製品名Anti-TLS/FUS antibody
    TLS/FUS 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TLS/FUS
  • 特異性We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: IHC-P, WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • 免疫原

    A region between residues 400 and 450 of human TLS/FUS

  • ポジティブ・コントロール
    • HeLa nuclear lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Detects a band of approximately 75 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.
  • 組織特異性Ubiquitous.
  • 関連疾患Note=A chromosomal aberration involving FUS is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3.
    Note=A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG.
    Defects in FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving FUS is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein.
    Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.
  • 配列類似性Belongs to the RRM TET family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾Arg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 75 kDa DNA pairing protein antibody
    • 75 kDa DNA-pairing protein antibody
    • ALS6 antibody
    • Amyotrophic lateral sclerosis 6 antibody
    • fus antibody
    • FUS CHOP antibody
    • Fus like protein antibody
    • FUS_HUMAN antibody
    • FUS1 antibody
    • Fused in sarcoma antibody
    • Fusion (involved in t(12;16) in malignant liposarcoma) antibody
    • Fusion derived from t(12;16) malignant liposarcoma antibody
    • Fusion gene in myxoid liposarcoma antibody
    • Heterogeneous nuclear ribonucleoprotein P2 antibody
    • hnRNP P2 antibody
    • hnRNPP2 antibody
    • Oncogene FUS antibody
    • Oncogene TLS antibody
    • POMp75 antibody
    • RNA binding protein FUS antibody
    • RNA-binding protein FUS antibody
    • TLS antibody
    • TLS CHOP antibody
    • Translocated in liposarcoma antibody
    • Translocated in liposarcoma protein antibody
    see all

Anti-TLS/FUS antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labelling TLS/FUS with ab70381 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-TLS/FUS antibody (ab70381) at 0.05 µg/ml

    Lane 1 : HeLa whole cell lysate at 10 µg
    Lane 2 : HeLa whole cell lysate at 50 µg

    Developed using the ECL technique

    Observed band size : 75 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
  • Detection of Human TLS/FUS by Immunoprecipitiaton. ab70381 used at 3µg/10cm plate for IP in lane 2, other antibodies used in lanes 1, 3, 4, 5 & 6. Control IgG used in lane 5. Lanes 1-5 - cells from one half of one 10cm plate of of HeLa whole cell lysate, and lanes 6 & 7 100µg of HeLa whole cell lysate used. ab70381 used at 0.02µg/ml in WB. Detection: Chemiluminescence with an exposure time of 5 seconds. Band size 58kDa.
  • ab70381 (1µg/ml) staining TLS in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab70381 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70381, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TLS/FUS antibody (ab70381) 使用論文

This product has been referenced in:
  • Tsuiji H  et al. Spliceosome integrity is defective in the motor neuron diseases ALS and SMA. EMBO Mol Med 5:221-34 (2013). Read more (PubMed: 23255347) »
  • Murakami T  et al. ALS mutations in FUS cause neuronal dysfunction and death in Caenorhabditis elegans by a dominant gain-of-function mechanism. Hum Mol Genet 21:1-9 (2012). IHC (PFA fixed) ; Human . Read more (PubMed: 21949354) »

See all 2 Publications for this product

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