製品の概要

  • 製品名Anti-TLS/FUS antibody
    TLS/FUS 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TLS/FUS
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Dog, Non Human Primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human TLS/FUS.

    (Peptide available as ab31610.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in nuclear extracts from HeLa and Jurkat cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab23439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 75 kDa).
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.
  • 組織特異性Ubiquitous.
  • 関連疾患Note=A chromosomal aberration involving FUS is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3.
    Note=A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG.
    Defects in FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving FUS is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein.
    Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.
  • 配列類似性Belongs to the RRM TET family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾Arg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 75 kDa DNA pairing protein antibody
    • 75 kDa DNA-pairing protein antibody
    • ALS6 antibody
    • Amyotrophic lateral sclerosis 6 antibody
    • fus antibody
    • FUS CHOP antibody
    • Fus like protein antibody
    • FUS_HUMAN antibody
    • FUS1 antibody
    • Fused in sarcoma antibody
    • Fusion (involved in t(12;16) in malignant liposarcoma) antibody
    • Fusion derived from t(12;16) malignant liposarcoma antibody
    • Fusion gene in myxoid liposarcoma antibody
    • Heterogeneous nuclear ribonucleoprotein P2 antibody
    • hnRNP P2 antibody
    • hnRNPP2 antibody
    • Oncogene FUS antibody
    • Oncogene TLS antibody
    • POMp75 antibody
    • RNA binding protein FUS antibody
    • RNA-binding protein FUS antibody
    • TLS antibody
    • TLS CHOP antibody
    • Translocated in liposarcoma antibody
    • Translocated in liposarcoma protein antibody
    see all

Anti-TLS/FUS antibody 画像

  • All lanes : Anti-TLS/FUS antibody (ab23439) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Observed band size : 75 kDa


    Exposure time : 8 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab23439 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • Anti-TLS/FUS antibody (ab23439) at 1/1000 dilution + Human 293T cell - whole cell lysate at 30000 cells

    Secondary
    An HRP Goat polyclonal to Rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa (possible non-specific binding),50 kDa (possible non-specific binding).

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk; 1% BSA for 16 hours at 4°C

    See Abreview

Anti-TLS/FUS antibody (ab23439) 使用論文

This product has been referenced in:
  • Joardar A  et al. PPAR gamma activation is neuroprotective in a Drosophila model of ALS based on TDP-43. Hum Mol Genet 24:1741-54 (2015). WB . Read more (PubMed: 25432537) »
  • Shen W  et al. 2'-Fluoro-modified phosphorothioate oligonucleotide can cause rapid degradation of P54nrb and PSF. Nucleic Acids Res 43:4569-78 (2015). Read more (PubMed: 25855809) »

See all 10 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - nuclear (hESC)
Gel Running Conditions Non-reduced Denaturing (4-20%)
Loading amount 20 µg
Specification hESC
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Dr. Miguel Fidalgo

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投稿 Nov 13 2015

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T Cells)
Loading amount 30000 cells
Specification 293T Cells
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step 5% Milk, 1% BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Feb 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HEK293 overexpressing HA-tagged FUS)
Total protein in input 2e+006 cells
Specification HEK293 overexpressing HA-tagged FUS
Immuno-precipitation step Other - Dynabeads
Username

Abcam user community

Verified customer

投稿 Oct 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 30 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Oct 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount 50 µg
Specification Mouse embryonic fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 15 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"