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Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human TLS/FUS.
(Peptide available as ab31610.)
Our Abpromise guarantee covers the use of ab23439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 75 kDa).|
|IP||Use at an assay dependent concentration.|
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab23439 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
This image is courtesy of an anonymous AbreviewBlocking Step: 5% Milk; 1% BSA for 16 hours at 4°C