製品の概要

  • 製品名Anti-TLR9 antibody [M9.D6] (Biotin)
    TLR9 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [M9.D6] to TLR9 (Biotin)
  • 標識Biotin
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    A peptide derived from the extracellular portion of Mouse TLR9.

  • ポジティブ・コントロール
    • Fixed and permeabilized Mouse TLR9 transfected 293 cells.
  • 特記事項


    TLR9 is not detected by flow cytometry using this antibody on RBC lysed Mouse splenocytes stained for intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Further studies are needed to determine the relationship between mRNA expression and protein detection.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • 一次抗体 備考TLR9 is not detected by flow cytometry using this antibody on RBC lysed Mouse splenocytes stained for intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Further studies are needed to determine the relationship between mRNA expression and protein detection.
  • ポリ/モノモノクローナル
  • クローン名M9.D6
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.25-0.5µg for 105-8 cells. A final volume of 100 µl is recommended for staining the cell sample.



ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 組織特異性Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • 配列類似性Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • 細胞内局在Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 289 antibody
    • CD289 antibody
    • TLR 9 antibody
    • TLR9 antibody
    • TLR9_HUMAN antibody
    • Toll like receptor 9 antibody
    • Toll like receptor 9 isoform A precursor antibody
    • Toll like receptor 9 isoform B antibody
    • Toll-like receptor 9 antibody
    see all

Anti-TLR9 antibody [M9.D6] (Biotin) 画像

  • Staining of fixed and permeabilized Mouse TLR9 transfected 293 cells with 0.25 µg of Rat IgG2a Isotype Control Biotin (open histogram) or 0.25 µg of ab95742 (filled histogram), followed by Streptavidin PE. Total cells were used for analysis.

Anti-TLR9 antibody [M9.D6] (Biotin) (ab95742) 使用論文

This product has been referenced in:
  • Fukata M  et al. The myeloid differentiation factor 88 (MyD88) is required for CD4+ T cell effector function in a murine model of inflammatory bowel disease. J Immunol 180:1886-94 (2008). Flow Cyt, ICC/IF . Read more (PubMed: 18209086) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"