製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TLR9
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to 15 amino acids from an internal sequence of human TLR9.

  • ポジティブ・コントロール
    • Jurkat cells, Jurkat cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 115 kDa.
ICC/IF Use a concentration of 2 µg/ml.

ターゲット情報

  • 機能
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 組織特異性
    Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • 配列類似性
    Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • 細胞内局在
    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 289 antibody
    • CD289 antibody
    • TLR 9 antibody
    • TLR9 antibody
    • TLR9_HUMAN antibody
    • Toll like receptor 9 antibody
    • Toll like receptor 9 isoform A precursor antibody
    • Toll like receptor 9 isoform B antibody
    • Toll-like receptor 9 antibody
    see all

画像

  • Lane 1 : Anti-TLR9 antibody (ab52967) at 0.5 µg/ml
    Lane 2 : Anti-TLR9 antibody (ab52967) at 1 µg/ml
    Lane 3 : Anti-TLR9 antibody (ab52967) at 2 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 115 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
  • Fixed with 4%-PFA.Immunocytochemical analysis of Jurkat cells using ab52967 at 2 µg/ml.

参考文献

This product has been referenced in:
  • Eichholz K  et al. Immune-Complexed Adenovirus Induce AIM2-Mediated Pyroptosis in Human Dendritic Cells. PLoS Pathog 12:e1005871 (2016). WB ; Human . Read more (PubMed: 27636895) »
  • Vegna S  et al. NOD1 Participates in the Innate Immune Response Triggered by Hepatitis C Virus Polymerase. J Virol 90:6022-35 (2016). WB . Read more (PubMed: 27099311) »

See all 2 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (liver, hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
liver, hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Aug 03 2015

Thank you for contacting us.

My colleague got back to me and we have now re-published ab52967.
Could you please just quickly confirm that I will send you 1 vial of ab52967 free of charge to replace ab134368?
The estimated delivery date...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (CD4+ T cells)
Loading amount
20 µg
Specification
CD4+ T cells
Treatment
anti-CD3/CD28 for 3 days
Gel Running Conditions
Reduced Denaturing (10% SDS)
Blocking step
gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jan 13 2009

Thank you for your patience. I have enquired at the originator of the product but unfortunately, the amino acid sequence is proprietary information and cannot be disclosed. I am sorry I can't be of more assistance in this occasion but should yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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