Anti-TLR7 抗体 [EPR2088(2)] (ab124928)

製品の概要

  • 製品名Anti-TLR7 antibody [EPR2088(2)]
    TLR7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2088(2)] to TLR7
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TLR7 (C terminal).

  • ポジティブ・コントロール
    • WB: Raji, Daudi and Ramos cell lysates. IHC-P: Human tonsil tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 121 kDa).

For unpurified use at 1/300.

IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/30.

  • 追加情報Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • 組織特異性Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
    • 配列類似性Belongs to the Toll-like receptor family.
      Contains 27 LRR (leucine-rich) repeats.
      Contains 1 TIR domain.
    • 細胞内局在Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
    • Information by UniProt
    • 参照データベース
    • 別名
      • PRO285 antibody
      • TLR 7 antibody
      • Tlr7 antibody
      • TLR7_HUMAN antibody
      • Toll like receptor 7 antibody
      • Toll-like receptor 7 antibody
      • UNQ248 antibody
      see all

    Anti-TLR7 antibody [EPR2088(2)] 画像

    • All lanes : Anti-TLR7 antibody [EPR2088(2)] (ab124928) at 20 µg (unpurified)

      Lane 1 : Ramos cell lysate
      Lane 2 : Daudi cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size : 121 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-TLR7 antibody [EPR2088(2)] (ab124928) at 1300 (purified)

      Lane 1 : Ramos cell lysate
      Lane 2 : Daudi cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 121 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-TLR7 antibody [EPR2088(2)] (ab124928) at 1/1000 dilution (unpurified)

      Lane 1 : Raji cell lysates
      Lane 2 : Ramos cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 121 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with purified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-TLR7 antibody [EPR2088(2)] (ab124928) 使用論文

    ab124928 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (8)
    Sample Human Tissue lysate - whole (Heart)
    Specification Heart
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 Nov 14 2013

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
    Sample Human Tissue sections (Spleen)
    Specification Spleen
    Permeabilization No
    Fixative Paraformaldehyde
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    投稿 Nov 12 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (peripheral blood cells)
    Loading amount 30 µg
    Specification peripheral blood cells
    Gel Running Conditions Reduced Denaturing (MES buffer, 4-12% Bis-Tris Gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
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    投稿 Sep 17 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"