製品の概要

  • 製品名
    Anti-TLR5 antibody [19D759.2]
    TLR5 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [19D759.2] to TLR5
  • 特異性
    This antibody recognizes an intracellular epitope in the cytoplasmic domain of TLR5.
  • アプリケーション
    適用あり: WB, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, IP, ICCmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide: SSKDFTWVQN ALLKHLDTQY SDQNRFNLCF EERDFVPGEN RIANIQDAIW NSRKIVCLVS RHFLRDGWCL EAFSYAQGRC LSDLNSALIM VVVGSLSQY QL conjugated to KLH, corresponding to amino acids 700-800 of Human TLR5.

  • ポジティブ・コントロール
    • Ramos and Raw cell lysate.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab168382 as a replacement.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 103 kDa.
Flow Cyt Use 2µg for 106 cells. Intracellular



ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Antigen retrieval with citrate buffer pH 6.
IHC-Fr Use a concentration of 10 µg/ml. (see Miller et al).
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 組織特異性
    Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
  • 関連疾患
    Systemic lupus erythematosus 1
  • 配列類似性
    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • 翻訳後修飾
    Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
    Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ10052 antibody
    • MGC126430 antibody
    • MGC126431 antibody
    • SLEB1 antibody
    • TIL 3 antibody
    • TIL3 antibody
    • TLR 5 antibody
    • Tlr5 antibody
    • TLR5_HUMAN antibody
    • Toll like receptor 5 antibody
    • Toll like receptor 5 precursor antibody
    • Toll-like receptor 5 antibody
    • Toll/interleukin 1 receptor like protein 3 antibody
    • Toll/interleukin-1 receptor-like protein 3 antibody
    see all

画像

  • All lanes : Anti-TLR5 antibody [19D759.2] (ab13876) at 2 µg/ml

    Lane 1 : Ramos cell lysate
    Lane 2 : Raw cell lysate


    Predicted band size : 103 kDa
  • Intracellular FACS analysis of TLR5 in 106 Ramos cells using (A) 2µg of isotype control and (B) 2µg of ab13876
  • ab13876 staining TLR5 in Human stomach tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 23°C; antigen retrieval was by heat mediation in citrate buffer (10mM, pH 6). Samples were incubated with primary antibody (10 µg/ml) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab13876 staining TLR5 in Human stomach tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (10 µg/ml in blocking buffer) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Yamada K  et al. Upregulation of Toll-like receptor 5 expression in the conjunctival epithelium of various human ocular surface diseases. Br J Ophthalmol N/A:N/A (2014). Human . Read more (PubMed: 24820048) »
  • Dill-McFarland KA  et al. Hibernation alters the diversity and composition of mucosa-associated bacteria while enhancing antimicrobial defence in the gut of 13-lined ground squirrels. Mol Ecol 23:4658-69 (2014). Squirrel . Read more (PubMed: 25130694) »

See all 6 Publications for this product

レビューと Q&A

Thank you for contacting us. An image of immunofluorescence can be found obn the website under the Abreviews section for ICC/IF. This product has not yet been used in immunofluorescence in a publication. The use of this product in ICC/IF in human or mo...

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I would like to confirm that both ab13875 and ab13876 recognise an intracellular portion of TLR5 and therefore need permeabilization. I'm afraid we do not have other antibodies tested in FACS against TLR5. However, ab1654 is raised against aa151-1...

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Thanks for your email. I'm not an expert in this area so I'm afraid that I'm unable to recommend anything specific. Doing a quick Pubmed search, the following reference may be useful for you: Toll-like receptor 5-mediated corneal epithelial inflamma...

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Thank you for your enquiry. We unfortunately do not have a conjugated TLR5 antibody. To see if another company may have what you are looking for, you can click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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