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Synthetic peptide corresponding to Human TLR4 aa 420-435.
Our Abpromise guarantee covers the use of ab47093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. PubMed: 21540291|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Flow Cytometry: Cell surface analysis using ab47093. Mouse macrophage cells were probed using 1 ug of TLR4 antibody (red) and 1 ug of isotype control (PE-conjugated secondary) antibody (green).
WB analysis of TLR4 using ab47093 at 2 ug/ml on partial recombinant mouse TLR4 protein.