製品の概要

  • 製品名Anti-TLR4 antibody
    TLR4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TLR4
  • 特異性TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate.
  • アプリケーション適用あり: ICC/IF, Electron Microscopy, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to TLR4 aa 39-56.
    Sequence:

    CMDQKLSKVPDDIPSSTK

  • ポジティブ・コントロール
    • WB: Partial recombinant mouse TLR4 (extracellular portion plus His-tag), RAW cell lysate, Daudi cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 16959900
Electron Microscopy Use at an assay dependent concentration. PubMed: 24489676
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75-80 kDa (predicted molecular weight: 96 kDa). Recommended lysis buffer: 10 mM Tris, pH8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi (tetrasodium Pyrophosphate)(supplemented with protease inhibitor cocktail).

ターゲット情報

  • 機能Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • 組織特異性Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • 関連疾患Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • 配列類似性Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • ドメインThe TIR domain mediates interaction with NOX4.
  • 翻訳後修飾N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

Anti-TLR4 antibody 画像

  • Analysis of TLR4 in mouse kidney tissue using ab13867 at 5µg/ml.



  • Predicted band size : 96 kDa

    Western blot analysis of TLR4 using ab13867 at 2 ug/ml on A) 0.1 ug/lane partial recombinant mouse TLR4 protein and B) RAW cell lysate and C) Daudi cell lysate. Western blot analysis of TLR4 using ab13867 at 2 ug/ml on A) 0.1 ug/lane partial recombinant mouse TLR4 protein and B) RAW cell lysate (ab7187) and C) Daudi cell lysate (ab3951).

Anti-TLR4 antibody (ab13867) 使用論文

This product has been referenced in:
  • Zhang Q  et al. Toll-like receptor 4 contributes to acute kidney injury after cardiopulmonary resuscitation in mice. Mol Med Rep 14:2983-90 (2016). Mouse . Read more (PubMed: 27510583) »
  • Tsai JC  et al. The role of calpain-myosin 9-Rab7b pathway in mediating the expression of Toll-like receptor 4 in platelets: a novel mechanism involved in a-granules trafficking. PLoS One 9:e85833 (2014). Electron Microscopy ; Human . Read more (PubMed: 24489676) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Mouse Tissue sections (frozen sections from mouse embryo)
Specification frozen sections from mouse embryo
Permeabilization Yes - triton-x 0.1% in TBS
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 03 2014

Thank you for calling Abcam.

I have sent you a vial of ab29545, HeLa cell lysate to use as a positive control.

Please let me know if there is anything else I can help you with, or if continue to experience any issue with our antibod...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Lungs)
Loading amount 30 µg
Specification Lungs
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Feb 26 2009

I'm sorry to hear you are experiencing problems with ab13867. The problems you are experiencing could be due to a number of reasons, such as low levels of TLR4 in your samples (due to the transfection procedure or the extraction procedure) and there...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"