Anti-TLR4 抗体 [76B357.1] (FITC) (ab45126)


  • 製品名Anti-TLR4 antibody [76B357.1] (FITC)
    TLR4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [76B357.1] to TLR4 (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • アプリケーション適用あり: Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Sheep, Horse, Cow, Cat, Pig, Chimpanzee, Baboon, Chinese Hamster
  • 免疫原

    Synthetic peptide corresponding to Human TLR4 aa 100-200.
    Database link: O00206

  • ポジティブ・コントロール
    • Ramos or THP1



Our Abpromise guarantee covers the use of ab45126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 2-5µg for 106 cells. ab18427-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.


  • 機能Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • 組織特異性Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • 関連疾患Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • 配列類似性Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • ドメインThe TIR domain mediates interaction with NOX4.
  • 翻訳後修飾N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

Anti-TLR4 antibody [76B357.1] (FITC) 画像

  • Immunofluorescence analysis of 16HBE14o- cells, staining TLR4 (red) with ab45126. Cells were fixed with paraformaldehyde for 10 min and permeabilized with 0.2% Triton X-100 in PBS for 10 min before incubating with primary antibody for 1 hour.
  • FACS analysis of cell surface TLR4 in human monocyte-derived dendritic cells using ab45126 at 1/200 (2.5 µg). Black and green open histograms represent unstained cells and isotype control (anti-human CD14 FITC) respectively. Blue open histogram is ab45126 anti-TLR4 stained dendritic cells. Data courtesy Anna Manlapat, National Institutes of Health

Anti-TLR4 antibody [76B357.1] (FITC) (ab45126) 使用論文

This product has been referenced in:
  • Ambarus CA  et al. Soluble Immune Complexes Shift the TLR-Induced Cytokine Production of Distinct Polarized Human Macrophage Subsets towards IL-10. PLoS One 7:e35994 (2012). Flow Cyt ; Human . Read more (PubMed: 22563430) »
  • Gries M  et al. Response of Toll-like receptors in experimental Guillain-Barré syndrome: a kinetic analysis. Neurosci Lett 518:154-60 (2012). Read more (PubMed: 22579825) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Sheep Cell (Macrophage)
Permeabilization No
Gating Strategy SSC FSC
Specification Macrophage
Fixation Paraformaldehyde

Mr. Jifan Yang

Verified customer

投稿 Feb 23 2016

From the antibodies you have been looking at, I would suggest ab45126 would be most suitable depending on the application you hope to use the antibody in. The antibody ab95563 is directed against the mouse Toll-like receptor 4 (TLR4)/MD-2. The hom...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Application Western blot
Sample Human Cell lysate - whole cell (Colon cancer (tissue lysate), U2OS (cell lysate) a)
Loading amount 20 µg
Specification Colon cancer (tissue lysate), U2OS (cell lysate) a
Gel Running Conditions Reduced Denaturing (Pierce precise protein gel 4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Antibody Solutions

Verified customer

投稿 Apr 06 2011