製品の概要

  • 製品名Anti-TLR3 antibody [TLR3.7] (Biotin)
    TLR3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TLR3.7] to TLR3 (Biotin)
  • 標識Biotin
  • アプリケーション適用あり: Flow Cyt, WB, IHC-Fr, Functional Studies, ICC/IF, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Dog, Human
  • 免疫原

    Human Flag-tagged TLR3 stably expressed by Ba/F3 cells.

  • ポジティブ・コントロール
    • Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab17264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/50. Predicted molecular weight: 100 kDa.
IHC-Fr 1/50.
Functional Studies Use at an assay dependent concentration.  Monoclonal antibody TLR3.7 inhibits dsRNA-induces IFN-beta production
ICC/IF 1/50.
IP 1/50.
IHC-P 1/50.

ターゲット情報

  • 機能Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 組織特異性Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
  • 関連疾患Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
  • 配列類似性Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 TIR domain.
  • ドメインds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
  • 翻訳後修飾Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
  • 細胞内局在Endoplasmic reticulum membrane. Endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD283 antibody
    • CD283 antigen antibody
    • IIAE2 antibody
    • TLR 3 antibody
    • Tlr3 antibody
    • TLR3_HUMAN antibody
    • Toll Like Receptor 3 antibody
    • Toll-like receptor 3 antibody
    see all

Anti-TLR3 antibody [TLR3.7] (Biotin) (ab17264) 使用論文

This product has been referenced in:
  • Matsumoto M  et al. Subcellular localization of Toll-like receptor 3 in human dendritic cells. J Immunol 171:3154-62 (2003). ICC/IF, Flow Cyt, IP ; Human . Read more (PubMed: 12960343) »
  • Matsumoto M  et al. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem Biophys Res Commun 293:1364-9 (2002). Read more (PubMed: 12054664) »

See all 3 Publications for this product

Product Wall

I heard back from our lab that the most recent lot of ab12085 had an endotoxin level of 0.02EU/ml. The ab17264 has not been tested for endotoxin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"