製品の概要

  • 製品名
    Anti-TLR3 antibody [TLR3.7] (Biotin)
    TLR3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TLR3.7] to TLR3 (Biotin)
  • 標識
    Biotin
  • アプリケーション
    適用あり: Flow Cyt, WB, IHC-Fr, Functional Studies, ICC/IF, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Dog, Human
  • 免疫原

    Human Flag-tagged TLR3 stably expressed by Ba/F3 cells.

  • ポジティブ・コントロール
    • Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab17264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/50. Predicted molecular weight: 100 kDa.
IHC-Fr 1/50.
Functional Studies Use at an assay dependent concentration.  Monoclonal antibody TLR3.7 inhibits dsRNA-induces IFN-beta production
ICC/IF 1/50.
IP 1/50.
IHC-P 1/50.

ターゲット情報

  • 機能
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 組織特異性
    Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
  • 関連疾患
    Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
  • 配列類似性
    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 TIR domain.
  • ドメイン
    ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
  • 翻訳後修飾
    Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
  • 細胞内局在
    Endoplasmic reticulum membrane. Endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD283 antibody
    • CD283 antigen antibody
    • IIAE2 antibody
    • TLR 3 antibody
    • Tlr3 antibody
    • TLR3_HUMAN antibody
    • Toll Like Receptor 3 antibody
    • Toll-like receptor 3 antibody
    see all

画像

  • Flow cytometric detection of human TLR3 on THP-1 cells. Red line represents the isotype control and the black line represents ab17264 at 10 μg/ml.

参考文献

This product has been referenced in:
  • Matsumoto M  et al. Subcellular localization of Toll-like receptor 3 in human dendritic cells. J Immunol 171:3154-62 (2003). ICC/IF, Flow Cyt, IP ; Human . Read more (PubMed: 12960343) »
  • Matsumoto M  et al. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem Biophys Res Commun 293:1364-9 (2002). Read more (PubMed: 12054664) »

See all 2 Publications for this product

レビューと Q&A

I heard back from our lab that the most recent lot of ab12085 had an endotoxin level of 0.02EU/ml. The ab17264 has not been tested for endotoxin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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