製品の概要

  • 製品名Anti-TLR2 antibody [TL2.1]
    TLR2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TL2.1] to TLR2
  • アプリケーション適用あり: ICC/IF, Flow Cyt, IP, Neutralising, WB, Functional Studiesmore details
  • 種交差性
    交差種: Dog, Human, Cynomolgus Monkey, Rhesus monkey, Marmoset (common)
  • 免疫原

    Recombinant human TLR2 produced by the CHO/CD14 reporter cell line.

  • 特記事項

    Ab9100 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 - 10 µg/ml.

Fix with PBS/2% formalin for 15 min at 4°C; permeabilize with acetone, 10 min, -20°C (see Flo et al J Leukoc Biol which used a conjugated form of the antibody).

Flow Cyt Use 2-3µg for 105 cells.

Untreated and 0.4 % formaldehyde fixed cells can be used; do not permeabilize the cells.

ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration. See Lien et al.
WB 1/50.

Abcam recommends using non-reducing conditions.

Functional Studies Use at an assay dependent concentration.

ターゲット情報

  • 機能Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
  • 組織特異性Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
  • 配列類似性Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • 翻訳後修飾Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD282 antibody
    • CD282 antigen antibody
    • TIL 4 antibody
    • TIL4 antibody
    • TLR 2 antibody
    • TLR2 antibody
    • TLR2_HUMAN antibody
    • Toll like receptor 2 antibody
    • Toll like receptor 2 precursor antibody
    • Toll-like receptor 2 antibody
    • Toll/interleukin 1 receptor like 4 antibody
    • Toll/interleukin 1 receptor like protein 4 antibody
    • Toll/interleukin receptor like protein 4 antibody
    • Toll/interleukin-1 receptor-like protein 4 antibody
    see all

Anti-TLR2 antibody [TL2.1] 画像

  • All lanes : Anti-TLR2 antibody [TL2.1] (ab9100) at 1 µg/ml

    Lane 1 : THP1
    Lane 2 : THP1 + LPS

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution

    Performed under reducing conditions.

    This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was ran at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked with 2% BSA for 60 minutes before being incubated with ab9100 (anti-TLR2 antibody; 1 ug/mL) for 18 hours at 4°C. Antibody binding was detected using ab97040 (HRP-labelled goat anti-mouse IgG)  at  1:50,000 dilutions for 1 hour at room temperature.Visualisation was conducted using ECL development solution ab133406.

    This gel was run under reducing conditions resulting in weak signal. We recommend customers to use non-reducing conditions.

  • ab9100 staining TLR2 in human U937 cells by Immunocytochemistry. Cells were fixed with paraformaldehyde and blocking with 2% BSA was done for 30 minutes at 270C. Samples were incubated with primary antibody (1/50) for 1 hour at 27°C. An Alexa Fluor®568-conjugated goat polyclonal to mouse IgG (H&L) was used at dilution at 1/50 as secondary antibody.

    See Abreview

  • TLR2 staining on human peripheral blood mononuclear cells by using ab9100. Green: CD3+-cells; Red: CD14+ cells

Anti-TLR2 antibody [TL2.1] (ab9100) 使用論文

This product has been referenced in:
  • Hwang SH  et al. Toll like receptor 3 & 4 responses of human turbinate derived mesenchymal stem cells: stimulation by double stranded RNA and lipopolysaccharide. PLoS One 9:e101558 (2014). Flow Cyt ; Human . Read more (PubMed: 25004159) »
  • Pyysalo MJ  et al. The connection between ruptured cerebral aneurysms and odontogenic bacteria. J Neurol Neurosurg Psychiatry N/A:N/A (2013). IHC-P ; Human . Read more (PubMed: 23761916) »

See all 14 Publications for this product

Product Wall

Thank you for your inquiry.

I can confirm that the storage temperature of our products can be found on the individual datasheets.

Short term storage is usually two weeks. If the products is not used within two weeks of purchase, we ...

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I am very pleased to hear you would like to accept our offer and test ab9100, ab17044, and ab117645 in rabbit. Each code will give you 1 free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forrabbit and incl...

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Thank you for getting back to me and for getting in touch with BioConnect. I will let you know if I don't hear anything from them shortly, and we will figure out the vial of ab9669. I'll also send you a confirmation email after I work everything out wi...

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Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 12 2014

Application Flow Cytometry
Sample Pig Cell (freshly isolated PBMC)
Specification freshly isolated PBMC
Gating Strategy living singlets
Permeabilization No
Username

Korneel Grauwet

Verified customer

投稿 Aug 01 2014

Thank you for your enquiry regarding.

I can confirm that ab9100 has been tested for functional and neutralizing assay whilst the other antibody has not (ab8376).

I regret to inform you that we have not conducted any studies to deter...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - whole (Brain)
Loading amount 25 µg
Specification Brain
Gel Running Conditions Reduced Non-Denaturing (Native) (4-20% gradient)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Apr 13 2012

Thank you for sending that email.

Unfortunately, none of the protocol information or data came through in the email. Could you please resend your protocol information and data, so that I can try and help resolve the issues you are having. Read More

Thank you for your reply, and I apologize for this inconvenience.

I certainly understand that ab9858 is not as useful to you as ab73680 would be. I can issue a credit to you.

The vial of ab8020 should be arriving shortly, please kee...

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Thank you again for your patience.

I apologize, but ab73680 is out of stock, and it may not be put back into stock for several months as recent lots have not passed quality control testing. Would you be interested in trying another vial of ab...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"