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Our Abpromise guarantee covers the use of ab11864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab11864 staining TLR2 in Mouse primary macrophage by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Acetone for 5 minutes at -20°C and blocked with 1% BSA for 60 minutes at 25°C. Samples were incubated with primary antibody (1/150 in PBS + 1% BSA) for 2 hours at 25°C. A Cy5®-conjugated Goat anti-rat IgG monoclonal (1/500) was used as the secondary antibody.