製品の概要

  • 製品名Anti-TLE 1 antibody
    TLE 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to TLE 1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human TLE 1 (NP_005068.2)

  • ポジティブ・コントロール
    • TLE 1 transfected 293T cell lysate, Human liver tissue lysate and Hela cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
  • 組織特異性In all tissues examined, mostly in brain, liver and muscle.
  • 配列類似性Belongs to the WD repeat Groucho/TLE family.
    Contains 6 WD repeats.
  • ドメインWD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.
  • 翻訳後修飾Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1.
    Ubiquitinated by XIAP/BIRC4.
  • 細胞内局在Nucleus. Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E(Sp1) homolog antibody
    • Enhancer of split groucho 1 antibody
    • Enhancer of split groucho like protein 1 antibody
    • Enhancer of split groucho-like protein 1 antibody
    • ESG antibody
    • ESG1 antibody
    • GRG1 antibody
    • TLE1 antibody
    • TLE1_HUMAN antibody
    • Transducin like enhancer of split 1 (E(sp1) homolog Drosophila) antibody
    • Transducin like enhancer of split 1 antibody
    • Transducin like enhancer of split 1 homolog of Drosophila E(sp1) antibody
    • Transducin like enhancer protein 1 antibody
    • Transducin-like enhancer protein 1 antibody
    see all

Anti-TLE 1 antibody 画像

  • Anti-TLE 1 antibody (ab89782) at 1 µg/ml + human liver tissue lysate at 50 µg

    Predicted band size : 83 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TLE 1 antibody (ab89782) at 1 µg/ml

    Lane 1 : TLE 1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 83 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • ab89782, at 10µg/ml, staining TLE 1 in HeLa cells by Immunofluorescence.

Anti-TLE 1 antibody (ab89782) 使用論文

This product has been referenced in:
  • Brunquell C  et al. TLE1 Is an Anoikis Regulator and Is Downregulated by Bit1 in Breast Cancer Cells. Mol Cancer Res 10:1482-95 (2012). WB . Read more (PubMed: 22952044) »

See 1 Publication for this product

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab89782.

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I actually managed to find your order for ab89782 and so I have therefore gone ahead ans refunded the cost of this antibody (please see the details below), since it did not work as we stated it should on the datashee...

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Thank you for contacting Abcam.

I am sorry that you are experiencing issues with ab89782 in western blot. Under our Abpromise, we do guarantee this antibody, for 6 months, to work in western blot against human samples.

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I am very pleased to hear you would like to accept our offer and test ab89782 in IHC. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC and include this code in the Addit...

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Thank you very much for your interest in ab89782.

To our knowledge, ab89782 has not been tested in IHC. Therefore, I can offer a discount off a future purchase if you buy ab89782 now, test it inIHC and submit feedback to us in the form of an...

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Thank you for contacting Abcam and for your interest in our products.

I have confirmed that the antibody ab89782 has been validated in WB for human tissue and is therefore guaranteed by our Abpromise. This states that we will replace any pro...

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