製品の概要

  • 製品名Anti-TJP2 antibody
    TJP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TJP2
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 993-1042 (EPQKAPSRPYQDTRGSYGSDAEEEEYRQQLSEHSKRGYYGQSARYRDTE L) of Human TJP2 (NP_963923).

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab81679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 134 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

ターゲット情報

  • 機能Plays a role in tight junctions and adherens junctions.
  • 組織特異性This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.
  • 関連疾患Defects in TJP2 are involved in familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
  • 配列類似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • 細胞内局在Cell junction > adherens junction. Cell membrane. Cell junction > tight junction. Nucleus. Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C9DUPq21.11 antibody
    • DFNA51 antibody
    • DUP9q21.11 antibody
    • Friedreich ataxia region gene X104 (tight junction protein ZO-2) antibody
    • MGC26306 antibody
    • PFIC4 antibody
    • Tight junction protein 2 antibody
    • Tight junction protein ZO 2 antibody
    • Tight junction protein ZO-2 antibody
    • TJP2 antibody
    • X104 antibody
    • ZO 2 antibody
    • ZO-2 antibody
    • ZO2 antibody
    • ZO2_HUMAN antibody
    • Zona occludens 2 antibody
    • Zona occludens protein 2 antibody
    • Zonula occludens protein 2 antibody
    see all

Anti-TJP2 antibody 画像

  • Anti-TJP2 antibody (ab81679) at 1 µg/ml ( in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 134 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <40 kDa. We are unsure as to the identity of these extra bands.

Anti-TJP2 antibody (ab81679) 使用論文

ab81679 has not yet been referenced specifically in any publications.

Product Wall

Western blot

Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Mouse Cell lysate - whole cell (C2C12 cells and Mouse Embryonic Fibroblasts)
Specification C2C12 cells and Mouse Embryonic Fibroblasts
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Jun 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"